Plasma was obtained via the centrifugation of sodium citrate tubes at 3000 rpm for 15 min at room temperature. FVIII:C and FVIII:Ag were measured via a one-stage clotting assay [49 (link)] and via enzyme immunoassay, respectively, using the Asserachrom VIII:Ag kit (Stago, Asnières sur Seine, France) (normal range for both tests: 51–147%). VWF:RCo and VWF:Ag were evaluated using the von Willebrand Ristocetin Cofactor (normal range 41–160% and 53–168% for O and non-O blood groups, respectively) and an automated immunoassay on ACL TOP analyzer (Instrumentation Laboratory, Milan, Italy), respectively (normal range: 40–165% and 55–169% for O and non-O blood groups, respectively). ADAMTS13 activity was measured using FRETS-VWF73 [50 (link)] (normal range: 45–138%). The activity of antithrombin (AT), protein C (PC), and protein S (PS) were measured via chromogenic (AT, PC) and clotting (PS) assays (Instrumentation Laboratory, Milan, Italy) using an ACL TOP analyzer (normal range: 82–112%, 65–160%, and 58–114%, respectively).
Acl top analyzer
Manufactured by Werfen
Sourced in United States, Italy
The ACL TOP analyzer is a laboratory instrument designed for automated coagulation testing. It performs a variety of coagulation assays, including prothrombin time (PT), activated partial thromboplastin time (aPTT), and other specialized tests. The analyzer provides reliable and consistent results, supporting clinical decision-making in hematology and hemostasis laboratories.
Automatically generated - may contain errors
Lab products found in correlation
Enzyme linked immunosorbent assay, by Enzyme Research (3 mentions)
Enoxaparin, by Sanofi (1 mentions)
Zymutest pai 1 antigen, by Hyphen Biomed (1 mentions)
Zymutest pai 1 activity, by Hyphen Biomed (1 mentions)
Au680 analyzer, by Beckman Coulter (1 mentions)
Cobas e411 analyzer, by Roche (1 mentions)
Hemosil reagents, by Werfen (1 mentions)
10 protocols using acl top analyzer
1
Hemostatic Factor Measurement Protocol
2
Heparin and LMWH Anticoagulant Potency Evaluation
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All fractions were stored frozen (−20°C) at 1 mg/mL in Aqua-Dest. Heparin (A3004,0001, 196 IU/mg; Applichem, Darmstadt, Germany) and LMWH (enoxaparin, 100 mg/mL, Sanofi-Aventis, Frankfurt, Germany) were stored according to the manufacturer's instructions for use. Dilutions in human pool plasma were used with the following concentrations (first dilution step to 100 µg/mL in 0.9% NaCl) (
n = 6): 0; 3.125; 6.25; 12.5; 25; and 50 µg/mL. The activated partial thromboplastin time (aPTT; SyntASil TM; reference range: 25–38 seconds) and anti-Xa activity (Liquid anti-Xa TM; reference range 0 IU/mL) were measured with commercially available assays according to the manufacturer's instructions (Werfen, Munich, Germany) and were analyzed on the ACL TOP analyzer (Werfen, Munich, Germany).
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All fractions were stored frozen (−20°C) at 1 mg/mL in Aqua-Dest. Heparin (A3004,0001, 196 IU/mg; Applichem, Darmstadt, Germany) and LMWH (enoxaparin, 100 mg/mL, Sanofi-Aventis, Frankfurt, Germany) were stored according to the manufacturer's instructions for use. Dilutions in human pool plasma were used with the following concentrations (first dilution step to 100 µg/mL in 0.9% NaCl) (
n = 6): 0; 3.125; 6.25; 12.5; 25; and 50 µg/mL. The activated partial thromboplastin time (aPTT; SyntASil TM; reference range: 25–38 seconds) and anti-Xa activity (Liquid anti-Xa TM; reference range 0 IU/mL) were measured with commercially available assays according to the manufacturer's instructions (Werfen, Munich, Germany) and were analyzed on the ACL TOP analyzer (Werfen, Munich, Germany).
3
Biomarker Panel for COVID-19 Prognosis
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C-reactive protein (CRP), ferritin and creatinine were measured on the Alinity IC analyzer (Abbott Diagnostics, Rungis, France). Procalcitonin (PCT) from Thermo Fisher was quantified on the Kryptor™ analyzer. D-dimers (D-dimers HS 500 IL) were quantified on the ACL TOP analyzer (Werfen, le Pré-Saint-Gervais, France). These parameters were considered if they were quantified within 24 h before or after PCR. CRP, ferritin, creatinine, PCT and D-dimers were available for 761, 415, 819, 253 and 480 patients, respectively.
4
Coagulation Factor IX Assessment
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Peripheral blood was collected from patients and their related family members using vacuum blood collection tubes containing 0.129 mol/L sodium citrate to obtain platelet-poor plasma. Coagulation factors activity levels, including FIX, were assessed using a one-stage clotting method on an automated ACL TOP analyzer (Instrumentation Laboratory, United States). FIX antigen levels were measured using an enzyme-linked immunosorbent assay following the manufacturer's instructions (Enzyme Research Laboratories, South Bend, Indiana, United States). Additional thrombophilia tests, including the activity levels of protein C (PC), protein S (PS), antithrombin III, and lupus anticoagulant, were performed as described previously.
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24 (link)
5
Plasma Biomarker Measurement Protocol
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Platelet poor plasma was obtained by centrifugation of citrated blood at 2500 g for 20 min at room temperature. Aliquots of citrated plasma, 1 ml each, were stored, shortly after collection, at -80°C until PAI-1 antigen, PAI-1 activity, TAFI assay were performed.
D-dimer concentrations were measured in citrated plasma by the d-dimer HS kit on ACL TOP analyzer (Instrumentation Laboratory, IL, USA). PAI-1 antigen was analyzed in duplicate by the ELISA kit ZYMUTEST PAI-1 Antigen (HYPHEN Biomed France). PAI-1 activity was measured in duplicate by the ELISA kit ZYMUTEST PAI-1 Activity (HYPHEN Biomed France). TAFI antigen was analyzed in duplicate by the IMUCLONE TAFI ELISA (Sekisui Diagnostics USA).
D-dimer concentrations were measured in citrated plasma by the d-dimer HS kit on ACL TOP analyzer (Instrumentation Laboratory, IL, USA). PAI-1 antigen was analyzed in duplicate by the ELISA kit ZYMUTEST PAI-1 Antigen (HYPHEN Biomed France). PAI-1 activity was measured in duplicate by the ELISA kit ZYMUTEST PAI-1 Activity (HYPHEN Biomed France). TAFI antigen was analyzed in duplicate by the IMUCLONE TAFI ELISA (Sekisui Diagnostics USA).
6
Comprehensive Laboratory Analyses in Clinical Center
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Laboratory analyses of CRP (Turbidimetric method, Beckman Coulter AU680 analyzer), ESR (Westergren method, Vacuette ESR analyzer), fibrinogen concentration (Clauss method, ACL TOP analyzer by Instrumentation Laboratory), lipid profile (cholesterol, triglycerides, HDL, and LDL, all by Beckman Coulter AU680 analyzer), rheumatoid factor (RF) (turbidimetric method, Beckman Coulter AU680 analyzer), and anti-citrullinated protein antibodies (ACPA) (Roche electrochemiluminescence immunoassay, Cobas e411 analyzer) were performed in the Central Laboratory of the Clinical Center Kragujevac [11 (link)].
7
Coagulation Analysis of Citrated Human Blood
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Sterile human blood in sodium citrate was purchased from the Rockland Immunochemicals, Inc (cat # R214-0050, Pottstown, PA 19464, USA). Plasma was prepared by centrifugation for 10 min at 2000× g. In vitro activity assays were performed on ACLTOP analyzer manufactured by Instrumentation Laboratory, which is a type of coagulation analyzer commonly used in clinical laboratories to measure clotting times and assess coagulation parameters. The ACLTOP analyzer is designed to perform a wide range of coagulation tests, including Factor II activity, prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen, and other related assays. ACLTOP analyzer uses HemosIL reagents developed by Instrumentation Laboratory company (Bedford, MA, USA) to perform coagulation tests.
8
Coagulation Markers Determination Protocol
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Prothrombin time/INR, fibrinogen and D-dimer were measured using the ACL TOP analyzer (Instrumentation Laboratory-IL, Werfen Group).
Q.F.A. Thrombin (Bovine) kit was used for the quantitative determination of fibrinogen, based on the Clauss method, in human citrated plasma on the IL Coagulation Systems.
The quantitative determination of D-dimer in human citrated plasma was determined by the automated latex enhanced immunoassay HemosIL D-Dimer HS (0020007700).
D-dimer classes were divided as follows: <500, 500–3,000, 3,000–7,000, and >7,000 ng/mL. The cut-off values of 3,000 and 7,000 ng/mL were chosen according to the proposal of ISTH about the proper values for moderate (2 points) and severe (3 points) increase in the ISTH overt-DIC score (14 (link)).
Q.F.A. Thrombin (Bovine) kit was used for the quantitative determination of fibrinogen, based on the Clauss method, in human citrated plasma on the IL Coagulation Systems.
The quantitative determination of D-dimer in human citrated plasma was determined by the automated latex enhanced immunoassay HemosIL D-Dimer HS (0020007700).
D-dimer classes were divided as follows: <500, 500–3,000, 3,000–7,000, and >7,000 ng/mL. The cut-off values of 3,000 and 7,000 ng/mL were chosen according to the proposal of ISTH about the proper values for moderate (2 points) and severe (3 points) increase in the ISTH overt-DIC score (14 (link)).
9
Plasma ADAMTS13 and VWF Measurement
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Plasma ADAMTS13 activity was measured using fluorescence resonance energy transfer on plasma samples, as previously described [21] . VWF antigen (VWF:Ag) and FVIII coagulant activity (FVIII:C) were measured using an automated immunoassay and one-stage clotting assay on ACL TOP analyzer (Instrumentation Laboratory Italy), respectively.
10
Coagulation Factor Assessment Protocol
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Samples were collected in vacuum tubes containing 0.109 mol/L sodium citrate. Platelet-poor plasma was obtained after two cycles of centrifugation at 20°C for 15 minutes at 2000 × g (to achieve a platelet count <10 × 10 9 /L), and then stored at -80°C. Standard coagulation tests were performed using the ACL TOP analyzer (Instrumentation Laboratory). Levels of FIX and FXI activity were measured in a clotting-based assay using lyophilized, deficient plasma from Siemens (Siemens Healthcare Diagnostics SAS) or Werfen (Instrumentation Laboratory). AT activity was determined using a chromogenic assay (Stachrom® ATIII, Diagnostica Stago SAS). PC and PS anticoagulant activities were determined in specific clotting-based assays (Staclot® PC and Staclot® PS, respectively; Diagnostica Stago SAS). The results were interpreted according to the age-appropriate reference interval for each assay.
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