Quantikine and duoset elisa kits

Manufactured by R&D Systems

Quantikine and Duoset ELISA kits are in-vitro diagnostic assay products manufactured by R&D Systems. They are designed to quantitatively measure specific proteins, cytokines, or other analytes in a variety of sample types. The kits utilize the enzyme-linked immunosorbent assay (ELISA) technique to detect and measure the target analyte present in the samples.

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Lab products found in correlation

Goat anti mouse ige, by Southern Biotech (1 mentions) Clonotyping elisa kits, by Southern Biotech (1 mentions) Tmb substrate solution, by R&D Systems (1 mentions) Facscanto 2, by BD (1 mentions) Fcap array software, by BD (1 mentions)

3 protocols using quantikine and duoset elisa kits

Cytokine and Chemokine Measurement in OVA-Induced Lung Inflammation

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To measure the levels of IL-2, IL-4, IL-5, IL-13, CCL2, CCL11, CCL17, CCL22, CXCL9, CXCL10, CXCL12, IL-1α, IL-1β, IL-6, and TNF-α and IFN-γ in the cell culture supernatant, BALF and lung homogenate, we used Quantikine and Duoset ELISA kits from R&D systems according to the manufacturer's instructions. OVA-specific IgE was measured by an ELISA kit from Biolegend. Measurements below the limit of detection were assigned a value of half the lower limit of detection for purposes of statistical analyses.

Zhou W., Zhang J., Goleniewska K., Dulek D.E., Toki S., Newcomb D.C., Cephus J.Y., Collins R.D., Wu P., Boothby M.R, & Peebles RS J.r. (2016). PGI2 suppresses proinflammatory chemokine expression, CD4 T cell activation, and STAT6-independent allergic lung inflammation. Journal of immunology (Baltimore, Md. : 1950), 197(5), 1577-1586.

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Quantification of Allergy Biomarkers in BAL Fluid

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The levels of IL-5 and IL-13 in BAL fluid were measured by Quantikine and Duoset ELISA kits (R&D Systems) according to the manufacturer’s instructions. Total IgE was determined by the Clonotyping ELISA kits (Southern Biotech). Ovalbumin-specific IgE in mouse sera was quantified as following. Immulon 2HB plates were coated with 2 μg ovalbumin in bicarbonate buffer (pH 9.6) per well overnight at 4 °C and blocked with 1% BSA. Serum samples were diluted in 0.1% Tween 20-PBS and incubated for 2 hours at room temperature. OVA-specific-IgE was detected by using goat-anti-mouse IgE conjugated with HRP (Southern Biotech). Plates were developed with TMB substrate solution (R&D Systems) and reactions were stopped with 1N HCl. Absorbance values at OD450 were measured. Concentrations of ovalbumin-specific IgE were determined using a standard curve made with serum from hyper-immunized mice.

Zhou W., Goleniewska K., Zhang J., Dulek D.E., Toki S., Lotz M.T., Newcomb D.C., Boswell M.G., Polosukhin V.V., Milne G.L., Wu P., Moore M.L., FitzGerald G.A, & Peebles RS J.r. (2014). COX inhibition abrogates aeroallergen-induced immune tolerance by suppressing PGI2 IP signaling. The Journal of allergy and clinical immunology, 134(3), 698-705.e5.

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Measuring Inflammatory Biomarkers in BALF and Plasma

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IL-6, vascular endothelial growth factor, MHC class I–related protein A, and semaphorin 7A (Sema7A) concentrations were measured by ELISA (Quantikine and Duoset ELISA kits; R&D Systems). BALF samples were undiluted and plasma samples were diluted 1:2. Sema7A concentrations were measured with a Cloud-Clone Corp ELISA Kit. IL-1β, IL-4, IL-8, IL-13, IL-17A, G-CSF, CXCL9, TNF, and IFN-γ concentrations were measured by cytometric bead array (CBA) (BD Biosciences). The standard and samples were applied to 96-well polypropylene Falcon V-bottom plates (Corning), and then capture beads diluent was added and incubation was performed for 1 hour. Next, detection reagent was applied to each well and incubated for 2 hours. After washing, samples were acquired on a flow cytometer (BD FACSCanto II). Analysis was performed using FCAP Array software (BD Biosciences). CXCL10 was measured by CBA using residual BALF samples after an additional freeze–thaw process.

Masuhiro K., Tamiya M., Fujimoto K., Koyama S., Naito Y., Osa A., Hirai T., Suzuki H., Okamoto N., Shiroyama T., Nishino K., Adachi Y., Nii T., Kinugasa-Katayama Y., Kajihara A., Morita T., Imoto S., Uematsu S., Irie T., Okuzaki D., Aoshi T., Takeda Y., Kumagai T., Hirashima T, & Kumanogoh A. (2022). Bronchoalveolar lavage fluid reveals factors contributing to the efficacy of PD-1 blockade in lung cancer. JCI Insight, 7(9), e157915.

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