C57bl 6 tg cag egfp 10sb j

C57BL/6, C57BL/6-Tg(CAG-EGFP)10sb/J, Pax7^tm1(cre)Mrc/J, B6.Cg-Pax7^{tm1(cre/ERT2)}Gaka/J, and C57BL/10ScSn-Dmd^mdx/J mice were purchased from Jackson Laboratories. p16^Ink4a knockout mice were obtained from the NCI Mouse Repository. Pax7-zsGreen transgenic mice were a kind gift from Dr. M. Kyba (University of Minnesota). Slug conditional knockout mouse line was generated in the current study. All compound genetically-engineered mice were a result of breeding the above strains followed by appropriate PCR-based genotyping. From 4 to 8-week-old male mice were used as adult mice in all the experiments, while 2-year-old male mice were used as aged mice. All the animal studies were approved by the Animal Care and Use Committee (approved protocol number: 16–111) and performed in compliance with the institutional guidelines of the University of Illinois at Chicago.

All animal procedures discussed in this manuscript for the ethical treatment of animals including handling, housing, surgeries, post-surgical monitoring, anesthesia and euthanasia were approved by the Institutional Animal Care and Use Committee at Temple University. eGFP (+) mice (C57BL/6-Tg(CAG-EGFP)10sb/J, The Jackson Laboratory) were genotyped under a fluorescent microscope. Pals1^f/f allele and Rx-Cre line were previously described [26 (link)]. PCR-based genotyping was performed using the primers as described. Pals1 CKO (PR; Pals1 ^f/f; Rx-Cre), heterozygote (Pals1^f/+; Rx-Cre) and wild type (WT) control littermates (mice not carrying Rx-Cre) were typically obtained from the crosses of heterozygotes and used for the assays. Swiss Webster (SW) mice potentially containing rd¹ allele (The Jackson Laboratory) were used as additional hosts for cell transplantation experiments.

C57BL/6, NOD/SCID immunodeficient, C57BL/6‐Tg(CAG‐EGFP)10sb/J, Pax7^tm1(cre)Mrc/J, ROSA^mT/mG, and C57BL/10ScSn‐Dmd^mdx/J mice were obtained from Jackson Laboratories. All experiments were performed with 4‐ to 8‐week‐old mice, and in compliance with the institutional guidelines of University of Illinois at Chicago.

C57BL/6 J (Jackson Laboratory 000664) were the wild type mice used for MuSC FACS purification for scRNAseq, muscle fiber isolation, and for all other in vitro experiments.
β-Actin GFP mice (C57BL/6-Tg(CAG-EGFP)10 sb/J, Jackson Laboratory 003291) were used to obtain GFP⁺ MuSCs for further implantation experiments. Mice lacking dystrophin expression (C57BL/10ScSn-Dmd^mdx/J, Jackson Laboratory 001801) were used for intraperitoneal MS023 injection and muscle physiology assessment. The experiments with the Dmd^mdx mice were performed at Sainte-Justine and were approved by the CHU Sainte-Justine Research Ethics Committee and performed in compliance with the Comité Institutionnel des Bonnes Pratiques Animales en Recherche (CIBPAR; approval number 2020–2668) in accordance with the Canadian Council on Animal Care guidelines. All other mouse husbandry and experiments were conducted in accordance with the Institutional Animal Care and Use Committee (IACUC) of McGill University. All animal procedures conducted at McGill were approved by the Animal Welfare Committee of McGill University (protocol #3506).