Procedures were conducted on protocols approved by the University of Illinois Institutional Animal Care and Use Committee. IL-1 receptor 1 knock out (KO), toll-like receptor (TLR-4) KO, IL-4 KO, and C57BL/6J [wild type (WT)] mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA) and bred in-house. All KO mice were on a C57BL/6 background. Mice were group housed (×8 cage) in standard shoebox cages (length 46.9 cm; width 25.4 cm; height 12.5 cm) and allowed water and Harlan chow (Indianapolis, IN, USA) ad libitum except where otherwise noted. Housing temperature (72°F) and humidity (45–55%) were controlled as was a 12/12 h reversed dark–light cycle (light = 2200–1000 h). After shipping, mice were allowed at least 1 week to adjust to the above conditions prior to experimental pre-conditioning. Mice were handled/scruffed for 1 week prior to behavioral testing and biomarker determinations. Animals were sacrificed using CO2 except for those in which corticosterone was measured, which were sacrificed using ketamine/xylazine. Different cohorts of mice were used for behavioral testing and biomarker studies. The total number of mice utilized was 430.
Il 4 ko
Manufactured by Jackson ImmunoResearch
Sourced in United States
IL-4 KO is a lab equipment product that functions as an interleukin-4 (IL-4) knockout reagent. It is designed to aid in the study of the role of IL-4 in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Tlr4ko, by Jackson ImmunoResearch (1 mentions)
C57bl 6j, by Jackson ImmunoResearch (1 mentions)
Rosa26 tdtomato mice, by Jackson ImmunoResearch (1 mentions)
Rag1 ko, by Jackson ImmunoResearch (1 mentions)
Ifngko, by Jackson ImmunoResearch (1 mentions)
C57bl 6 b6 mice, by Koatech (1 mentions)
Foxp3yfp cre, by Jackson ImmunoResearch (1 mentions)
Foxp3egfp creert2, by Jackson ImmunoResearch (1 mentions)
Pdgfrα creer mice, by Jackson ImmunoResearch (1 mentions)
4 hydroxytamoxifen h7904, by Merck Group (1 mentions)
Plx5622, by Plexxikon (1 mentions)
Pparγflox flox, by Jackson ImmunoResearch (1 mentions)
C57bl 6j wt, by Jackson ImmunoResearch (1 mentions)
B6 mrl faslpr j, by Jackson ImmunoResearch (1 mentions)
4 protocols using il 4 ko
1
Genetic Knockout Mice for Inflammatory Studies
2
Genetically Modified Mice for Immunology Research
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C57BL/6 (B6) mice (7 to 8 weeks old) were purchased from Koatech (Pyeongtaek, Korea). Crif1fl/fl mice were a generous gift from M. Shong (Chungnam University, Korea) and were crossed with Foxp3YFP-Cre (Jackson strain no. 016959) or Foxp3EGFP-cre-ERT2 (Jackson strain no. 016961) mice. Other purchased mice were B6 CD45.1 congenic (Jackson strain no. 002014), T-betfl/fl (Jackson strain no. 022741), Ifng KO (Jackson strain no. 002287), Il4 KO (Jackson strain no. 002253), Rag1 KO (Jackson strain no. 002216), and ROSA26tdtomato mice (Jackson strain no. 007909) mice. All of these Jackson strain mice were purchased from Jackson Laboratory. Gata3fl/fl mice were a gift from Professor G. Ryol Lee (Sogang University, Korea). All strains were maintained on a B6 background and crossed as necessary to generate the desired genotypes. Age- and gender-matched male and female mice between 4 and 8 weeks of age were used in the study. Littermates were randomly assigned to experimental groups. All mice were bred and maintained under specific pathogen–free conditions at the Clinical Research Institute, SNUH. All in vivo experiments were approved by the Institutional Animal Care and Use Committee of the Clinical Research Institute, SNUH, and were conducted in accordance with the relevant guidelines and regulations.
3
Selective OPC Depletion and Microglia/Macrophage Modulation
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C57BL/6J WT, IL-4 KO, PPARγflox/flox, and PDGFRαCreER mice were purchased from Jackson Laboratory (Bar Harbor, ME, United States). PDGFRαCreER(+/−)PPARγflox/flox mice were bred from PPARγflox/flox and PDGFRαCreER mice. The depletion of PPARγ in OPCs was induced in PDGFRαCreER(+/−)PPARγflox/flox mice (8-wk-old males) by intraperitoneal injection of 4-hydroxytamoxifen (H7904, Sigma, St. Louis, MO, USA, 0.1 mg in 100 μL corn oil, daily for 5 consecutive days). For microglia/macrophage depletion, PLX5622 (Plexxikon, Berkeley, CA, USA) was supplied to mice (9–10 wk old, 25–30 g body weight) in the diet (Research Diets, New Brunswick, NJ, USA) at 1,200 PPM (1,200 mg/kg of chow), starting 7 d prior to surgery and continuing until the end of experiments.
4
Genetically Modified Mice for Immunology Research
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Male and female BALB/c mice, wild-type ones, and mutants lacking either IL-4 (IL-4 KO; Jackson Laboratory online reference http://jaxmice.jax.org/strain/002496.html ) functional genes or an enhancer element in the promoter region of gene coding for the GATA-1 transcription factor [26 (link)], required for eosinophil-lineage determination (GATA-1 dbl KO or GATA-1 for short; Jackson Laboratory online reference http://jaxmice.jax.org/strain/005653.html ), and C57BL/6 mice, wild-type ones, and mutants lacking iNOS [33 (link), Jackson reference #002596] functional genes were bred at CECAL-FIOCRUZ (Rio de Janeiro, Brazil) and used at 6–8 weeks of age. Animal housing and handling followed procedures approved by the Institutional Committee on Ethical Handling of Laboratory Animals (Licenses CEUA #L002-09; CEUA-CCS-UFRJ #181-13). Mutant mice lacking functional IL17RA [39 (link)] or CD95 genes (B6.MRL-Faslpr/J; Jackson Laboratory online reference http://jaxmice.jax.org/strain/000482.html ), bred at the Department of Pharmacology, Ribeirão Preto Medical School, University of São Paulo, Brazil, along with the respective wild-type controls, were also used as approved by the Institutional Ethics Committee (CEUA-CCS-UFRJ #181-13).
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