Horseradish peroxidase hrp conjugated secondary antibodies for immunoblotting

Polyclonal anti-human IL-8 was obtained from R&D Systems (Minneapolis, MN, USA). IgG from goat serum was purchased from Sigma-Aldrich, Co. (St. Louis, MO, USA). Antibody to anti-p-JNK, anti-p-p38, anti-p38 and anti-IκB-α antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and anti-JNK, anti-p-IκB-α (ser32) was purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-tubulin antibody was purchased from Sigma-Aldrich, Co. Horseradish peroxidase (HRP)-conjugated secondary antibodies for immunoblotting were obtained from Santa Cruz Biotechnology. The NF-κB inhibitor BAY 11-7092, JNK inhibitor SP600125, p38 inhibitor SB203580 were purchased from Sigma-Aldrich, Co.

H₂O₂, N-acetyl-l-cysteine (NAC), and catalase were purchased from Sigma (St. Louis, MO, USA). PTP inhibitor IV (PTPi IV) was purchased from Calbiochem (San Diego, CA, USA). Recombinant glutathione S-transferase (GST)-SHP-2 and GST-CSK were purchased from Abnova (Taipei, Taiwan). Antibodies against caveolin-1, p-caveolin-1 (pY14) and SHP-2 were obtained from BD Transduction Laboratories (Lexington, KY, USA). Anti-CSK antibody and anti-FAK were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). p-SHP-2 Y542, p-SHP-2 Y580, Src, p-Src Y416, p-Src Y529, and p-FAK antibodies were obtained from Cell Signaling (Danvers, MA, USA). Tubulin antibody was obtained from Sigma. Horseradish peroxidase (HRP)-conjugated secondary antibodies for immunoblotting were obtained from Santa Cruz Biotechnology.

H₂O₂ and catalase were purchased from Sigma. Antibodies against p-ERK1/2, ERK1/2, p-Src Y419, p-Src Y530 and Src were obtained from Cell Signaling (Beverly, MA, USA). Anti-Csk, anti-p-p38, anti-p38, anti-p-JNK, anti-JNK antibodies were obtained from Santa Cruz Technology (Santa Cruz, CA, USA). Antibody against caveolin-1 was obtained from BD Biosciences (San Diego, CA, USA). Tubulin antibody was obtained from Sigma. All Horseradish peroxidase (HRP)-conjugated secondary antibodies for immunoblotting were obtained from Santa Cruz Technology. Alexa flour 568-conjugated rabbit secondary antibody was obtained from Life Technologies.