Recombinant human jagged 1

Manufactured by R&D Systems

Recombinant human Jagged-1 is a protein produced in a mammalian cell expression system. It is a member of the Jagged family of Notch ligands.

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Lab products found in correlation

1xb27 supplement, by Thermo Fisher Scientific (1 mentions) Recombinant murine egf, by Thermo Fisher Scientific (1 mentions) Recombinant murine noggin, by Thermo Fisher Scientific (1 mentions) Recombinant human r spondin1, by R&D Systems (1 mentions) N acetylcysteine, by Merck Group (1 mentions) 1xn2 supplement, by Thermo Fisher Scientific (1 mentions) Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions) Anti notch3, by R&D Systems (1 mentions) Anti dll4, by R&D Systems (1 mentions) Anti cd45 apc vio770, by Miltenyi Biotec (1 mentions) Gsi xii, by Merck Group (1 mentions) Goat igg pe, by R&D Systems (1 mentions) Anti jagged1 fitc, by R&D Systems (1 mentions) Anti dll3 pe, by R&D Systems (1 mentions) Anti notch1 pe, by BioLegend (1 mentions) Anti notch2 pe, by BioLegend (1 mentions) Anti notch3 pe, by BioLegend (1 mentions) Anti notch4 pe, by BioLegend (1 mentions) Anti dll1 pe, by BioLegend (1 mentions) Anti dll4, by BioLegend (1 mentions)

3 protocols using recombinant human jagged 1

Culturing Human Intestinal Organoids

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A thin film of liquid type I collagen (Advanced BioMatrix, San Diego, CA) was plated onto the bottom surface of 48-well Nucleon Delta-treated cell culture plate (Thermo Scientific, Waltham, MA) at a concentration of 100 ng/mL, incubated for 30 minutes, and then removed by aspiration. Human crypts were plated at a density of 500 crypts per well. These were grown as monolayers in Basic Medium with antibiotic-antimycotic (Invitrogen), 1 mM N-Acetylcysteine (Sigma), 100 ng/ml recombinant murine Noggin (PeproTech), 50 ng/ml recombinant murine EGF (PeproTech), 1xN2 supplement (Invitrogen), 1xB27 supplement (Invitrogen), 1 μg/ml recombinant human R-spondin 1 (R&D Systems, Minneapolis, MN), 10 μM Y-27632 inhibitor (Stemgent), 1 mM recombinant human Jagged-1 (R&D Systems), 5 μM CHIR99021 (GSK-3β inhibitor) (Stemolecule). Alternatively, crypts were suspended within Matrigel at a concentration of 100 crypts per 25 μl of Matrigel and plated in 3D on the 48-well Nucleon Delta-treated cell culture plate.

Rouch J.D., Scott A., Lei N.Y., Solorzano-Vargas R.S., Wang J., Hanson E.M., Kobayashi M., Lewis M., Stelzner M.G., Dunn J.C., Eckmann L, & Martín M.G. (2016). Development of Functional Microfold (M) Cells from Intestinal Stem Cells in Primary Human Enteroids. PLoS ONE, 11(1), e0148216.

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Multiparametric Analysis of Notch Signaling in Leukemia

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The antibodies used for FACS analysis were: mouse IgG2b-FITC, goat IgG-PE, anti-Jagged1-FITC, anti-Dll3-PE (all from R&D System, Minneapolis, MN), mouse IgG2a-PE, mouse IgG1κ-PE, mouse IgG1-Alexa Fluor 488,anti-Notch1-PE, anti-Notch2-PE, anti-Notch3-PE, anti-Notch4-PE, anti-Dll1-PE, anti-Dll4, anti-Bax-Alexa Fluor 488 (all from Biolegend, San Diego, CA) and rabbit anti-Bcl-2-FITC (DAKO). For leukemia cell identification we used anti-CD45-VioBlue, anti-CD45-APC-Vio770, anti-CD34-PerCP and anti-CD117-APC (all from MiltenyiBiotec, Germany). The antibodies employed for western blot analysis anti-Notch2, anti-Notch4 were from Santa Cruz (Biotechnology, Dallas, TX), anti-GAPDH and HRP conjugated secondary antibodies against mouse, rabbit or goat were from Sigma Aldrich. All the other antibodies used for Western blot were from Cell Signalling. Neutralizing antibodies,all used at a final concentration of 5 μg/ml, were: anti-Notch1, anti-Notch3,anti-Jagged1, anti-Jagged2, anti-Dll1 and anti-Dll4 (R&D Systems); anti-Notch-4 (Santa Cruz Biotechnology); anti-Dll3 (CST, Boston, MA). Recombinant human Jagged-1 and Jagged-2 were from R&D System. GSI-IX (DAPT) was purchased from Stemgent (Cambridge, MA) GSI-XII and SAHM1 were from Merck Millipore (Darmstadt, Germany). Cytarabine (Ara-C), Etoposide (Eto) and Idarubicin (Ida) were provided by Pharmacy Unit of the University Hospital of Verona.

Kamga P.T., Bassi G., Cassaro A., Midolo M., Di Trapani M., Gatti A., Carusone R., Resci F., Perbellini O., Gottardi M., Bonifacio M., Kamdje A.H., Ambrosetti A, & Krampera M. (2016). Notch signalling drives bone marrow stromal cell-mediated chemoresistance in acute myeloid leukemia. Oncotarget, 7(16), 21713-21727.

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TCDD and ITE Signaling Pathway

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2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) in dimethyl sulfoxide (DMSO) was purchased from Cambridge Isotopes Laboratory (Andover, MA). 2-(1H-Indol-3-ylcarbonyl)-4-thiazolecarboxylic acid methyl ester (ITE) was purchased from Tocris Bioscience (Bristol, United Kingdom). STATTIC and recombinant human JAGGED1 were purchased from R&D Systems (Minneapolis, MN). Non-targeting short interfering RNA (siRNA) (D-001810-01-20), ON-TARGETplus siRNA against AHR (J-004990-08-0010) and JAG1 (J-011060-11-0005) were purchased from GE Dharmacon (Lafayette, CO). The following supplies were purchased from ThermoFisher Scientific (Pittsburgh, PA): Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12), fetal bovine serum (FBS), phosphate buffered saline (PBS), penicillin/streptomycin (P/S), dimethyl sulfoxide (DMSO), TrypLE Express and Lipofectamine RNAiMAX. Sodium dodecyl sulfate (SDS), 30% acrylamide/bis solution, ammonium persulfate, Tween-20, 2-mercaptoethanol and polyvinylidene difluoride (PVDF) membranes were obtained from BIO-RAD (Hercules, CA). Cell lines were purchased from the American Type Culture Collection (Manassas, VA) and cultured in DMEM:F-12 supplemented with 10% FBS and P/S at 37 °C with 5% CO₂.

Piwarski S.A., Thompson C., Chaudhry A.R., Denvir J., Primerano D.A., Fan J, & Salisbury T.B. (2020). The putative endogenous AHR ligand ITE reduces JAG1 and associated NOTCH1 signaling in triple negative breast cancer cells. Biochemical pharmacology, 174, 113845.

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