Sq22536

Uric acid sodium was purchased from Sigma Aldrich (St. Louis, USA). Forskolin and SQ22536 were purchased from MedChemExpress (USA). Phorbol 12-myristate 13-acetate (PMA), propidium iodide (PI), 4’, 6-diamidino-2-phenylindole (DAPI), and Antifade Mounting Medium were provided by Keygen biotech (Nanjing, China). siRNA for transfection was obtained from Genepharma (Shanghai, China). Lipofectamine 2000 was derived from Invitrogen. Caspase-1 Detection Kit was supplied from ImmunoChemistry Technologies (USA). NLRP3 (bs-10021R), ASC (bs-6741R), caspase-1 (bs-10442R), and β-actin (bs-0061R) antibodies for western blot were obtained from Bioss (Beijing, China). NLRP3 (sc-34410) and ASC (sc-22514-R) primary antibodies used for immunofluorescence were obtained from Santa Cruz Biotechnology (USA). Donkey Anti-Goat IgG H&L (Alexa Fluor 488) (ab150129) and Donkey Anti-Rabbit IgG H&L (Alexa Fluor 647) (ab150075) second antibodies used for Immunofluorescence were obtained from Abcam (USA). All cell culture supplement components were obtained from Gibco (Waltham, MA, USA).

Tempol, apocynin, db-cAMP, SQ22536, vigabatrin, gabazine, yohimbine and clonidine were obtained from MedChemExpress (Monmouth Junction, NJ, USA). LPS, CGP35348, DEX and DETC were purchased from Sigma (St Louis, MO, USA). Rp-cAMP was purchased from Beyotime (Shanghai, China). apocynin and CGP35348 were dissolved in PBS containing 1% DMSO and the vehicle was used as a control. Other chemicals were dissolved in PBS. PBS was used as a control.

SQ22536, db-cAMP, H89, and asprosin were purchased from Med Chem Express (Monmouth Junction, NJ, USA). Tempol, apocynin, and NAC were obtained from Sigma (St. Louis, MO, USA). Asprosin and Tempol were dissolved in PBS, and other chemicals were dissolved in PBS containing 1% DMSO. Vehicles were used as controls.

Minimum essential medium, fetal bovine serum, penicillin/streptomycin and trypsin were purchased from Gibco (Grand Island, NY, United States). Allicin was provided by Ailexin (Urumuqi, China). Amiloride, CFTRinh-172, DIDS, forskolin (FSK), isobutylmethylxanthine (IBMX) were purchased from Sigma-Aldrich (Missouri, United States). SQ22536 was purchased from MedChemExpress (New Jersey, United States). NaCl, KCl, MgSO₄, NaHCO₃, KH₂PO₄, CaCl₂, glucose, and gluconate were purchased from Guangzhou Chemical Pharmaceutical Factory (Guangzhou, China). HEPES was purchased from Mbchem Technology (Guangzhou, China). The direct cAMP enzyme immunoassay kit (KGE002B) was purchased from R&D Systems (Minneapolis, MN, United States). The bicinchoninic acid protein assay kit (kw0014) was purchased from KWBIO (Beijing, China).