Protein lysate of hESCs was prepared in RIPA buffer and NuPAGE® LDS sample loading buffer and denatured for 10 min at 98°C. Samples were run on an SDS- PAGE 10% Bis-Tris gel (ThermoFisher) 60–80 min at 100V, transferred at 100V for 60–70 min, and blocked with 5% non-fat dried milk in 0.1%TBST (0.1% Tween in Tris Buffer Saline solution) for 1 hr. Primary antibody was added to blocking buffer and incubated at room temperature for 1 hr. Secondary antibodies were added after washing with TBST. The PierceTM ECL Western Blotting Substrate (ThermoScientific, 32109) was used on the membrane before film exposure. The primary antibodies used in this study include: rabbit-anti-TFAP2C (Abcam, 76007, 1:1000), mouse-anti-Histone 3 (Abcam, 10799, 1:8000). The secondary antibodies used include: Donkey-anti-goat HRP (Invitrogen, A15999, 1:2000), Sheep-anti-mouse HRP (GE Healthcare Life Sciences, NA931VS, 1:2000).
Donkey anti goat hrp
Manufactured by Thermo Fisher Scientific
Donkey-anti-goat HRP is a secondary antibody conjugated with horseradish peroxidase (HRP). It is used to detect and visualize goat primary antibodies in various immunoassays and immunohistochemical applications.
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Lab products found in correlation
2 protocols using donkey anti goat hrp
1
Quantifying TFAP2C and Histone 3 in hESCs
2
Quantifying TFAP2C and Histone 3 in hESCs
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Protein lysate of hESCs was prepared in RIPA buffer and NuPAGE® LDS sample loading buffer and denatured for 10 min at 98°C. Samples were run on an SDS- PAGE 10% Bis-Tris gel (ThermoFisher) 60–80 min at 100V, transferred at 100V for 60–70 min, and blocked with 5% non-fat dried milk in 0.1%TBST (0.1% Tween in Tris Buffer Saline solution) for 1 hr. Primary antibody was added to blocking buffer and incubated at room temperature for 1 hr. Secondary antibodies were added after washing with TBST. The PierceTM ECL Western Blotting Substrate (ThermoScientific, 32109) was used on the membrane before film exposure. The primary antibodies used in this study include: rabbit-anti-TFAP2C (Abcam, 76007, 1:1000), mouse-anti-Histone 3 (Abcam, 10799, 1:8000). The secondary antibodies used include: Donkey-anti-goat HRP (Invitrogen, A15999, 1:2000), Sheep-anti-mouse HRP (GE Healthcare Life Sciences, NA931VS, 1:2000).
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