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2 protocols using sodium l pyruvate

1

Neurotransmitter Receptor Agonist and Antagonist Assay

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Sodium L-lactate and sodium L-pyruvate were purchased from Sigma-Aldrich. (5S,10R)-(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine maleate (MK801 maleate) and N-methyl-D-aspartic acid (NMDA) were purchased from Tocris. NADH was obtained from Roche.
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2

Neural Stem Cell Differentiation Assay

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Sodium L-lactate, sodium L-pyruvate, temozolomide (TMZ), sodium β-hydroxybutyric acid, sesame oil, 5-Bromo-2′-deoxyuridine (BrdU), poly-L-ornithine, L-arginine, L-lysine, glucose, paraformaldehyde, DMSO, and saccharin were from Sigma-Aldrich (Switzerland). Corticosterone was from Tocris Bioscience (UK). β-Nicotinamide adenine dinucleotide (NADH) was from Roche (Switzerland). CellROX green reagent and 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI) were from Thermo Fisher Scientific (USA). Sodium acetoacetate was from BOC Sciences (USA). Penicillin and streptomycin were from Invitrogen (USA). SILAC advanced Dulbecco’s Modified Eagle’s Medium/F-12 flex media, N2 supplement, and mouse laminin were from Gibco (USA).
Anti-BrdU mouse monoclonal antibody (B44) was from BD Biosciences (USA). Goat anti-mouse Alexa Fluor 488 (A11029) and 594 (A11032) antibodies and goat anti-rabbit Alexa Fluor 488 (A11008) antibody were from Thermo Fisher Scientific (USA). Anti-NeuN rabbit monoclonal antibody (ab177487) was from Abcam (UK). Anti-SOX-2 rabbit polyclonal antibody (AB5603) and anti-Nestin mouse monoclonal antibody (MAB353) were from Sigma-Aldrich (Switzerland).
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