Anti phospho 4e bp1 ser65 antibody

Manufactured by Cell Signaling Technology

The Anti-phospho 4E-BP1 Ser65 antibody is a tool for detecting the phosphorylation of the 4E-BP1 protein at the serine 65 residue. This antibody can be used in various research applications to study the regulation and activity of 4E-BP1, which plays a role in the control of protein translation.

Automatically generated - may contain errors

Lab products found in correlation

Anti mtor antibody, by Cell Signaling Technology (2 mentions) To pro 3, by Thermo Fisher Scientific (2 mentions) Anti phospho mtor antibody, by Cell Signaling Technology (2 mentions) Whole blood anti human cd15 microbeads, by Miltenyi Biotec (2 mentions) Anti 4e bp1 antibody, by Cell Signaling Technology (2 mentions) Anti phospho 4e bp1 t37 46 antibody, by Cell Signaling Technology (2 mentions) Anti phospho 4e bp1 thr70 antibody, by Cell Signaling Technology (2 mentions) Anti human paf receptor and anti human igg isotype matched control antibodies, by Abcam (2 mentions) Anti il6rα primary antibody, by Santa Cruz Biotechnology (2 mentions) Anti β actin antibody, by Cell Signaling Technology (2 mentions) Platelet activating factor, by Avanti Polar Lipids (2 mentions) Media 199, by Lonza (2 mentions) Yl1 2, by Santa Cruz Biotechnology (1 mentions) Goat anti rabbit igg hrp linked antibody, by Cell Signaling Technology (1 mentions)

3 protocols using anti phospho 4e bp1 ser65 antibody

Platelet-activating Factor Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

We purchased the following reagents: Platelet-activating factor (Avanti Polar Lipids, Alabaster, AL); Whole Blood anti-human CD15 microbeads (Miltenyi Biotec, Auburn, CA); Media-199 (Lonza Biologics, Alpharetta, GA); TOPRO-3 (Molecular Probes, Eugene, OR); Rapamycin (Calbiochem, Burlington, MA). We purchased the following antibodies from Cell Signaling Technology (Danvers, MA): anti-mTOR antibody (#2972), anti-phospho mTOR antibody (#2971), anti-4E-BP1 antibody (#9452), anti-phospho 4E-BP1 T37/46 antibody (#9459), anti-phospho 4E-BP1 Ser65 antibody (#9451), anti-phospho 4E-BP1 Thr70 antibody (#9455), anti-β-actin antibody. Anti-human PAF-receptor and anti-human IgG isotype-matched control antibodies were purchased from Abcam (Cambridge, MA). The goat-anti-rabbit Alexa Fluor 488 secondary antibody was purchased from Life Technologies (Carlsbad, CA) and the anti-IL6Rα primary antibody from Santa Cruz Biotechnology (Dallas, TX).

Campbell R.A., Cody M.J., Manne B.K., Zimmerman G.A, & Yost C.C. (2019). Interleukin 6 receptor alpha expression in PMNs isolated from prematurely born neonates: Decreased expression is associated with differential mTOR signaling.. Pediatric research, 86(1), 55-62.

+ Open protocol

+ Expand

Diverse Antibodies for Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used in this study: rabbit monoclonal anti-phospho-PDH-E1α (Ser293) antibody (EPR12200: Abcam, Cat#ab177461), mouse monoclonal anti-PDH-E1α antibody (D-6: Santa Cruz Biotechnology, Cat#sc-377092), rat monoclonal anti-α-tubulin antibody (YL1/2: Santa Cruz Biotechnology, Cat#sc-53029), mouse monoclonal anti-actin antibody (AC-40: Sigma, Cat#A3853), mouse monoclonal anti-p38α MAPK antibody (L53F8: Cell Signaling, Cat#9228), rabbit polyclonal anti-COXⅣ antibody (proteintech, Cat#11242-1-AP), mouse monoclonal anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr404) antibody (E10: Cell signaling, Cat#9106), rabbit polyclonal anti-p44/42 MAPK (Erk1/2) antibody (Cell Signaling, Cat#9102), rabbit polyclonal anti-phospho-acetyl-CoA-carboxylase (Ser79) antibody (Cell Signaling, Cat#3661), rabbit monoclonal anti-acetyl-CoA-carboxylase antibody (C83B10: Cell Signaling, Cat#3676), rabbit polyclonal anti-phospho-Akt (Ser473) antibody (Cell Signaling, Cat#9271), rabbit monoclonal anti-Akt antibody (11E7: Cell Signaling, Cat#4685), and rabbit polyclonal anti-phospho-4E-BP1 (Ser65) antibody (Cell Signaling, Cat#9451). Goat anti-rabbit IgG, HRP-linked antibody (Cell Signaling, Cat#7074), horse anti-mouse IgG, HRP-linked antibody (Cell Signaling, Cat#7076), and goat anti-rat IgG, HRP-linked antibody (Cell Signaling, Cat#7077) were used as secondary antibodies.

Ikizawa T., Ikeda K., Arita M., Kitajima S., Soga T., Ichijo H, & Naguro I. (2022). Mitochondria directly sense osmotic stress to trigger rapid metabolic remodeling via regulation of pyruvate dehydrogenase phosphorylation. The Journal of Biological Chemistry, 299(2), 102837.

+ Open protocol

+ Expand

Platelet-activating Factor Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!