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Anti hla a2 fitc antibody

Manufactured by BioLegend

The Anti-HLA-A2 FITC antibody is a laboratory reagent used for the detection and identification of HLA-A2 antigen expression on cells. It is conjugated with the fluorescent dye FITC, allowing for detection and quantification of HLA-A2 positive cells through flow cytometry or other fluorescence-based techniques.

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2 protocols using anti hla a2 fitc antibody

1

Quantifying HLA-A2 pMHC Binding

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Streptavidin coated nanovials were functionalized with biotinylated HLA-A*02:01 restricted NY-ESO-1 pMHC by incubating at various concentrations (0, 20, 40, 80, 90, 100 μg/mL) and washed three times as described in Nanovial Functionalization section. Nanovials were reconstituted at a ten-fold dilution in Washing Buffer containing 2 μL of 100 μg/mL anti-HLA-A2 FITC antibody (Biolegend, 343304) and incubated for 30 minutes at 37°C. After washing three times with Washing Buffer, mean fluorescence intensity was measured by flow cytometry.
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2

Quantifying Cell Surface HLA-A2 Expression

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Cells were incubated with anti-HLA-A2-FITC antibody (BioLegend, cat. 343304) or anti-IgG2b-FITC isotype control antibody (BioLegend, cat. 401206) diluted at 1:50 in PBS for 30 min at 4°C. Cells were washed and resuspended in 50 μl of FACS buffer (PBS + 0.5% BSA + 4 Mm EDTA). Data was acquired using BD Accuri C6 platform (BD Biosciences) and represented as relative abundance in mean fluorescence intensity (MFI) relative to isotype/unstained ratio.
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