Body weights were weekly determined over the 12 weeks of the experimental study. Food intake was measured daily from week 13 to week 14 of age. For Insulin sensitivity tests, mice were injected with Insulin (0.4 IU/kg) i.p. and blood glucose determined at the indicated time points. For the GTTs, D-glucose (2g/kg) was injected intraperitoneally (i.p.) and blood glucose determined at the indicated time points. Both assays were performed at the end of the 12 weeks of feeding study. Mice were fasted for 4 hours. Blood glucose was measured using an automated blood glucose reader Glucometer (OneTouch® Ultra® 2 Glucometer, Lifescan). For BrdU (US Biological, B2850) treatment, BrdU was administered in the drinking water at 0.4 mg ml−1 for one week, starting after completion of the tamoxifen treatment, when mice were 7 week of age. BrdU water was refreshed every two days. At the end of the BrdU treatment, mice were kept for three more weeks in their respective diet groups. Mice were then sacrificed and the organs collected for further analysis.
B2850
Manufactured by US Biological
The B2850 is a laboratory equipment designed for biological research and analysis. It serves as a general-purpose centrifuge, capable of separating and concentrating various biological samples, such as cells, organelles, and molecules, based on their different densities. The core function of the B2850 is to facilitate the separation and purification of cellular components and biomolecules through the application of centrifugal force.
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Lab products found in correlation
3 protocols using b2850
1
Insulin Sensitivity and Glucose Tolerance Assays
2
Insulin Sensitivity and Glucose Tolerance Assays
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Body weights were weekly determined over the 12 weeks of the experimental study. Food intake was measured daily from week 13 to week 14 of age. For Insulin sensitivity tests, mice were injected with Insulin (0.4 IU/kg) i.p. and blood glucose determined at the indicated time points. For the GTTs, D-glucose (2g/kg) was injected intraperitoneally (i.p.) and blood glucose determined at the indicated time points. Both assays were performed at the end of the 12 weeks of feeding study. Mice were fasted for 4 hours. Blood glucose was measured using an automated blood glucose reader Glucometer (OneTouch® Ultra® 2 Glucometer, Lifescan). For BrdU (US Biological, B2850) treatment, BrdU was administered in the drinking water at 0.4 mg ml−1 for one week, starting after completion of the tamoxifen treatment, when mice were 7 week of age. BrdU water was refreshed every two days. At the end of the BrdU treatment, mice were kept for three more weeks in their respective diet groups. Mice were then sacrificed and the organs collected for further analysis.
3
BrdU Dosing Protocol for Cell Labeling
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