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Hiv 1 p24

Manufactured by Santa Cruz Biotechnology
Sourced in Canada

The HIV-1 p24 is a laboratory product used for the detection and quantification of the HIV-1 p24 antigen. The p24 antigen is a core protein of the human immunodeficiency virus type 1 (HIV-1) and its presence is an indicator of active viral replication.

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2 protocols using hiv 1 p24

1

Multicolor Flow Cytometry for HIV-1 Gag

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For flow cytometry, the anti-HLA-A2-APC (BB7.2), anti-CD4-FITC (OKT4), anti-CD3-PE (UCHT1), and anti-CD8-Pacific blue (RPA-T8) were purchased from BD Biosciences, the anti-MHC-I-APC(W6/32) purchased from eBioscience. For intracellular HIV-1 Gag (p24) staining, surface staining was performed, then followed by intracellular staining for HIV-1 p24 (Santa Cruz Biotechnology) with the transcription factor buffer set according to the manufacturer's protocol (BD Biosciences). Data were acquired on a BD FACS aria and were analyzed with FlowJo software (Tree Star, Ashland, OR).
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2

Exosome Characterization Antibody Panel

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BST-2, CD63, CD9, CD81, TSG101, LSP-1, LAMP-1, GW182, HIV-1 Reverse Transcriptase, HIV-1 p24, Actin and GAPDH antibodies were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Integrin β1, Ago2, TRBP-2 and Dicer antibodies were obtained from Cell Signaling Technology (Danvers, MA). MHC II antibody were obtained from Abcam (Cambridge, MA). LFA-1 antibody was obtained from R&D systems (Minneapolis, MN). Exosome-depleted FBS was obtained from System Biosciences (Palo Alto, CA).
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