ML-SA1, MK6–83, praziquantel, and serotonin were from Sigma-Aldrich (St. Louis MO). LysoTracker RED DND-99 was from Life Technologies, Inc. ML-SI3 was obtained from Enamine, code (s) EN 300–314172. Stock solutions were made in dimethyl sulfoxide (DMSO, ATCC, Manassas, VA) and subsequently diluted to an appropriate concentration in culture or recording media. All oligonucleotides were bought from Integrated DNA Technologies (IDT, Coralville, IA).
Mk6 83
Manufactured by Merck Group
Sourced in Sao Tome and Principe
The MK6-83 is a laboratory equipment product designed for general use in scientific research and analysis. It serves as a versatile tool for performing various tasks in a laboratory setting. The core function of the MK6-83 is to provide a reliable and consistent platform for conducting experiments and measurements. The specific details of its intended use are not included in this factual description.
Automatically generated - may contain errors
Lab products found in correlation
Ml sa1, by Merck Group (3 mentions)
A 769662, by Merck Group (2 mentions)
Ml si3, by Merck Group (2 mentions)
Bafilomycin a1, by Merck Group (2 mentions)
Ionomycin, by Merck Group (2 mentions)
Il 15, by Miltenyi Biotec (2 mentions)
Praziquantel, by Merck Group (1 mentions)
Serotonin, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by American Type Culture Collection (1 mentions)
Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions)
3 protocols using mk6 83
1
Fluorescent Probes for Cellular Imaging
2
TRPML1 Agonist and Antagonist Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The TRPML1 agonist MK6-83 (Sigma-Aldrich) was dissolved in DMSO and used at 20 μΜ unless specified otherwise. The TRPML1 antagonist ML-SI3 (enamine) was dissolved in DMSO and used at 50 μΜ. The chemical modulators ML-SA1, A-769662, bafilomycin A1, ionomycin, MGR1, and MGR2 were used as indicated in the figure legends, and all were obtained from Sigma-Aldrich. Clinical-grade recombinant human IL-2 (Proleukin) was obtained from Clinigen via Sykehusapoteket (no. 600373). IL-15 was used at 1 or 10 ng/ml (Miltenyi Biotec).
3
TRPML1 Agonist and Antagonist Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The TRPML1 agonist MK6-83 (Sigma-Aldrich) was dissolved in DMSO and used at 20 μΜ unless specified otherwise. The TRPML1 antagonist ML-SI3 (enamine) was dissolved in DMSO and used at 50 μΜ. The chemical modulators ML-SA1, A-769662, bafilomycin A1, ionomycin, MGR1, and MGR2 were used as indicated in the figure legends, and all were obtained from Sigma-Aldrich. Clinical-grade recombinant human IL-2 (Proleukin) was obtained from Clinigen via Sykehusapoteket (no. 600373). IL-15 was used at 1 or 10 ng/ml (Miltenyi Biotec).
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