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Apc conjugated anti gfpuv egfp antibody

Manufactured by R&D Systems
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The APC-conjugated anti-GFPuv/eGFP antibody is a lab equipment product designed for use in flow cytometry and other immunofluorescence applications. It binds to and labels GFPuv and eGFP proteins, enabling their detection and analysis.

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Lab products found in correlation

Percp cy5.5 conjugated mouse anti h2ax ps139, by BD (2 mentions) Pe conjugated anti human oct4 oct3 antibody, by STEMCELL (2 mentions) Facsaria 2, by BD (2 mentions)

2 protocols using apc conjugated anti gfpuv egfp antibody

1

Monitoring Pluripotency and Genomic Integrity

Check if the same lab product or an alternative is used in the 5 most similar protocols
To directly monitor the eGFP expression levels, the wild type or mutant POU5F1-eGFP cells were dissociated with Accutase and subjected to FACS analysis with BD FACSAria II. To examine the levels of HLA-C protein, the cells were stained with PE-conjugated antibody specifically recognizing HLA-C (Millpore, Cat#MABF233). To carry out immunostaining of eGFP, POU5F1, or γH2AX, the cells were fixed with 2% PFA for 30 minutes, followed by overnight permeabilization in Methanol at −20°C. The treated cells were stained with the antibodies. PerCP-cy5.5-conjugated mouse anti-H2AX(pS139) was purchased BD Biosciences (Cat#564718); PE-conjugated anti-human OCT4(OCT3) antibody was from STEMCELL Technologies (Cat# 60093PE.1) and APC-conjugated anti-GFPuv/eGFP antibody is available from R&D Systems (Cat# IC4240A)
Diao Y., Fang R., Li B., Meng Z., Yu J., Qiu Y., Lin K.C., Huang H., Liu T., Marina R.J., Jung I., Shen Y., Guan K.L, & Ren B. (2017). A tiling1deletion based genetic screen for cis-regulatory element identification in mammalian cells. Nature methods, 14(6), 629-635.
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2

Monitoring Pluripotency and Genomic Integrity

Check if the same lab product or an alternative is used in the 5 most similar protocols
To directly monitor the eGFP expression levels, the wild type or mutant POU5F1-eGFP cells were dissociated with Accutase and subjected to FACS analysis with BD FACSAria II. To examine the levels of HLA-C protein, the cells were stained with PE-conjugated antibody specifically recognizing HLA-C (Millpore, Cat#MABF233). To carry out immunostaining of eGFP, POU5F1, or γH2AX, the cells were fixed with 2% PFA for 30 minutes, followed by overnight permeabilization in Methanol at −20°C. The treated cells were stained with the antibodies. PerCP-cy5.5-conjugated mouse anti-H2AX(pS139) was purchased BD Biosciences (Cat#564718); PE-conjugated anti-human OCT4(OCT3) antibody was from STEMCELL Technologies (Cat# 60093PE.1) and APC-conjugated anti-GFPuv/eGFP antibody is available from R&D Systems (Cat# IC4240A)
Diao Y., Fang R., Li B., Meng Z., Yu J., Qiu Y., Lin K.C., Huang H., Liu T., Marina R.J., Jung I., Shen Y., Guan K.L, & Ren B. (2017). A tiling1deletion based genetic screen for cis-regulatory element identification in mammalian cells. Nature methods, 14(6), 629-635.
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