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Cells annexin 5 apoptosis detection kits

Manufactured by Thermo Fisher Scientific
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The Cells Annexin V Apoptosis Detection Kits are used to detect and quantify apoptosis in cell populations. The kits utilize Annexin V, a protein that binds to phosphatidylserine, and a viability dye to identify early and late apoptotic cells.

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Lab products found in correlation

Rnaase a, by Merck Group (2 mentions) Facscalibur, by BD (2 mentions)

2 protocols using cells annexin 5 apoptosis detection kits

1

Cell Cycle and Apoptosis Analysis

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Cells with different treatments were trypsinized, washed with PBS, and fixed in ice-cold 70% ethanol at 4 °C overnight. After being washed with PBS, cells were incubated with RNAase A (Sigma) in PBS for 30 min at 37 °C and then stained with 50 mg/ml propidium iodide. Cell cycle data were collected with FACS Calibur (Becton Dickinson) and analyzed with FlowJo software. Apoptosis of cells was analyzed with FACS using Cells Annexin V Apoptosis Detection Kits as per the manufacture’s standard procedures (Affymetrix eBioscience).
Yang S., Liu L., Cao C., Song N., Wang Y., Ma S., Zhang Q., Yu N., Ding X., Yang F., Tian S., Zhang K., Sun T., Yang J., Yao Z., Wu S, & Shi L. (2018). USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization. Nature Communications, 9, 1285.
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2

Cell Cycle Analysis and Apoptosis Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells with different treatments were trypsinized, washed with PBS and fixed in 70% ethanol at 4 °C overnight. After being washed with PBS, cells were incubated with RNAase A (Sigma) in PBS for 30 min at 37 °C and then stained with 50 mg ml−1 propidium iodide. Cell cycle data were collected with FACS Calibur (Becton Dickinson) and analysed with the FlowJo software. Apoptosis of cells was analysed with FACS using Cells Annexin V Apoptosis Detection Kits as per the manufacturer's standard procedures (Affymetrix eBioscience).
Li X., Song N., Liu L., Liu X., Ding X., Song X., Yang S., Shan L., Zhou X., Su D., Wang Y., Zhang Q., Cao C., Ma S., Yu N., Yang F., Wang Y., Yao Z., Shang Y, & Shi L. (2017). USP9X regulates centrosome duplication and promotes breast carcinogenesis. Nature Communications, 8, 14866.
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