The cells were stained with CFSE at a final concentration of 5 μM at 37 °C for 10 min and cultured in 12-well plates in RPMI medium with 10% FBS at a density of 60,000 cells per well for 48 h. Cells were then cultured with 0.1% DMSO or the compound at 37 °C for another 24 or 48 h. After treatment, the cells were collected and detected using flow cytometric analysis (FACSan FL1 channel, Becton Dickinson, Mountain View, CA, USA). Proliferation index was analyzed with ModFit LTTM 3.3 (Verity Software House, Topsham, ME, USA).
Modfit lttm 3
Manufactured by Verity Software House
Sourced in United States
ModFit LTTM 3.3 is a laboratory software application designed for cell cycle analysis. It provides tools for data acquisition, cell cycle modeling, and statistical analysis of flow cytometry data.
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Lab products found in correlation
2 protocols using modfit lttm 3
1
Cell Proliferation Assay with CFSE
2
CFSE-based Cell Proliferation Assay
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The cells were cultured in six-well plates in RPMI 1640 medium with 5% (v/v) FBS at a density of 50,000 cells per well and stained with CFSE for 48 h. Cells were then incubated with 0.1% DMSO or compound treatment for another 24 or 48 h. After the treatment, cell proliferation was detected by FACSan FL1 channel (Becton Dickinson, San Jose, CA, USA). Proliferation index was analyzed with software ModFit LTTM 3.3 (Verity Software House, Topsham, ME, USA) to quantify the inhibition of cell proliferation.
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