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3 protocols using chloroquine diphosphate

1

Evaluation of Antioxidant Compounds

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N-acetyl-L-cysteine (NAC), Chloroquine diphosphate (CQ), MDC, MK2206, SC79, DCFH-DA probe were obtained from MedChemExpress. 5-Aminolevulinic acid (ALA) was purchased from Sigma.
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2

Autophagy and Mitochondrial Dynamics Assay

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MTT was purchased from Sigma‐Aldrich (USA). Chloroquine diphosphate, E‐64, PKI‐402 and rapamycin were purchased from MedChemExpress (USA). The primary antibodies against LC3B, SQSTM1/p62, TFEB, PINK1, parkin, Fis1, Drp1, Mfn1 and Mfn2 were purchased from Abcam (USA), and LAMP1, beta‐actin, alpha‐tubulin, cathepsin B, cathepsin D and cytochrome c were purchased from Proteintech (USA).
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3

Cell Culture and FDA-Approved Drug Screening

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Vero-E6 (American Type Culture Collection [ATCC], CRL-1586), Vero (ATCC, CCL-81), BHK21-hACE2 1 cells were maintained in high glucose DMEM (SIGMA-ALDRICH) supplemented with 10% FBS (GIBCO), penicillin (100 IU/mL), streptomycin (100 μg/mL) in a 5% CO2 environment at 37°C and passaged every 2 days. In addition, the culture medium of BHK21-hACE2 contains puromycin (2 μg/mL). The FDA-approved drug library, including 1403 compounds (10 mM DMSO solutions), and Chloroquine diphosphate were bought from MedChemExpress (MCE).
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