Anti nlrp 3 antibody

Recombinant human GM-CSF and tumor necrosis factor-alpha (TNF-α) were purchased from PeproTech (Rocky Hill, NJ, USA). Anti-β-actin antibodies were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Anti-NLRP3 antibody was purchased from Merck Millipore (Billerica, MA, USA). Human IL-1β and caspase-1 (p20) enzyme-linked immunosorbent assay (ELISA) kits were purchased from R&D Systems (Minneapolis, MN, USA). Human IL-18 ELISA kits were purchased from MBL (Nagoya, Japan). Phospho-specific antibodies against JAK-1 (Tyr1022/1023), JAK-2 (Tyr1007/1008), STAT-5 (Tyr701), and STAT-3 (Tyr705) were purchased from Cell Signaling Technology (Beverly, MA, USA). Phospho-specific antibody against JAK3 (Tyr980) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-phospho-1κB-α (Ser32), anti-phospho-NF-κB p65 (Ser536), anti-IκBα, and anti-NF-κB antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-phospho-specific Asc (Tyr-144) antibody was purchased from ECM Biosciences (Versailles, KY, USA). Anti-caspase-1 antibody (14F468) was purchased from Novus Biologics (Littleton, CO, USA). Tofacitinib was purchased from Sigma-Aldrich (Tokyo, Japan).

Recombinant human GM-CSF was purchased from Peprotech (Rocky Hills, NJ). Anti-β-actin antibodies were purchased from Santa Cruz Biotechnology Inc. (Dallas, USA). Anti-NLRP-3 antibody was purchased from MERCK MILLIPORE (Billerica, MA USA). Human IL-1β and caspase-1 (p20) ELISA kits were purchased from R&D systems (Minneapolis, USA). Phospho-specific antibodies against JAK-2 (Tyr1007/1008), STAT-5 (Tyr701) and STAT-3 (Tyr705) were purchased from Cell Signaling Technology (Beverly, MA). Tofacitinib, baricinib and upadacitinib were purchased from Sigma-Aldrich (Tokyo, Japan).

Recombinant human SAA was purchased from Peprotech (Rocky Hills, NJ). The endotoxin levels were less than 0.1 ng/μg protein. Anti-β-actin antibodies were purchased from Santa Cruz Biotechnology Inc. (Dallas, USA). Anti-NLRP-3 antibody was purchased from MERCK MILLIPORE (Billerica, MA, USA). Human IL-1β and caspase-1 (p20) ELISA kits were purchased from R&D systems (Minneapolis, USA). Anti-phospho-NF-κB p65 (Ser536) antibody was purchased from Cell Signaling Technology (CST, Danvers, USA). Hydroxychloroquine, iberiotoxin (IBTX), lipopolysaccharide (LPS), and adenosine 5 triphosphate (ATP) were purchased from Sigma-Aldrich (Tokyo, Japan).