Human il 1β elisa set

To determine superoxide dismutase 1 (SOD1), IL-1β, and VEGF contents in HFDPC, the ab119520 -Superoxide Dismutase 1 Human ELISA kit (Abcam, Trumpington, Cambridge, UK), Human IL-1β ELISA Set (BD Biosciences, Franklin Lakes, NJ, USA), and Quantikine ELISA human VEGF (DVE00) kits (R&D Systems, Minneapolis, MN, USA) were used according to the manufacturer’s instructions. For H₂O₂ treatment the samples were treated for 20 h and then treated with 1000 μM H₂O₂ for 4 h, and then the supernatant was collected, and protein was measured. To determine VEGF contents in NIH3T3 fibroblasts, the Mouse VEGF (MMV00) Quantikine ELISA kits (R&D Systems, Minneapolis, MN, USA) were used according to the manufacturer’s instructions. NIH3T3 cells treated with samples for 24 h, and then the supernatant was collected, and protein was measured.