Polyglactin 910 4.0 thread

The animals were fasted for eight hours before the surgical procedure, sedated by the combination of 50 mg/kg intramuscular ketamine (Vetaset-Fort Dodge Saúde Animal Ltd., Campinas, SP, Brazil) and 5 mg/kg xylazine hydrochloride (Dopaser-Laboratório Calier do Brasil Ltd., Osasco, SP, Brazil), and received mepivacaine hydrochloride (0.3 mL/Kg, Scandicaine 2% with adrenaline 1:100,000, Septodont, SaintMaur-des-Fossés, France) as local anesthesia and for hemostasis of the operative field.
After sedation, the animal was positioned on a surgical table specially made for surgical procedures in rodents, favoring the maintenance of an open oral cavity and providing an adequate positioning for the exodontia procedure. After antisepsis of the area using topical polyvinylpyrrolidone iodine (PVPI 10%, Riodeine Degermante, Rioquímica, São José do Rio Preto, SP, Brazil), the extraction of the upper first molar was performed with the aid of a Hollenbeck 3S sculptor (Quinelato, Rio Claro, SP, Brazil). Diuresis, syndesmotomy, and then dislocation of the upper first molar were performed with the Hollenbeck 3S sculptor using the wedge and lever principle, and then elevation of the dental element (exodontia) was performed. Finally, the alveolar mucosa was sutured with Polyglactin 910 4.0 thread (Figure 2A–D) (Vicryl 4.0, Johnson & Johnson, New Brunswick, NJ, USA).

After anesthesia with xylazine hydrochloride (Xilazine-Coopers, Brazil, Ltd., Osasco, SP, Brazil) and ketamine hydrochloride (ketamine hydrochloride, injectable, Fort Dodge, Animal Health, Ltd., Campinas, SP, Brazil), the animals were immobilized on a surgical board in lateral decubitus a 1 cm incision was made in the flanks, and the subcutaneous tissue and then the peritoneum were divided by layers to access the abdominal cavity. The ovaries and uterine horns were then located and lacquered with Polyglactin 910 4.0 wire (Vicryl 4.0, Johnson & Johnson, New Brunswick, NJ, USA). After that, the ovaries were removed. For synthesis, suturing was performed in layers with Polyglactin 910 4.0 thread (Vicryl 4.0, Johnson & Johnson, New Brunswick, NJ, USA). The rats in the SHAM group underwent the same procedure, but only surgical exposure of the uterine horns and ovaries was performed, without their respective ligation and removal.