Immunohistochemistry was performed as previously described42 (link). Anti-α-smooth muscle actin (α-SMA) antibody (1:100, Dako) was used as a primary antibody. Western blotting was carried out as previously described43 (link). Anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (Merck Millipore) and anti-α-SMA were used as primary antibodies at a dilution of 1:1000.
Anti α smooth muscle actin α sma antibody
Manufactured by Agilent Technologies
Sourced in Denmark
The Anti-α-smooth muscle actin (α-SMA) antibody is a laboratory reagent used to detect and quantify the α-SMA protein, which is a component of the cytoskeleton in smooth muscle cells. This antibody can be used in various immunoassay techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to analyze the expression of α-SMA in biological samples.
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Lab products found in correlation
2 protocols using anti α smooth muscle actin α sma antibody
1
Immunohistochemistry and Western Blot Analysis
2
Immunohistochemical Analysis of Kidney Tissue
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For immunohistochemistry, the 2-µm sections were deparaffinized and soaked in methanol
containing 0.3% hydrogen peroxide for 30 min to block endogenous peroxidase activity. The
sections were then treated with 10% normal goat serum for 30 min to block nonspecific
reactions. Anti-α-smooth muscle actin (α-SMA) antibody (mouse polyclonal, Dako, Denmark;
diluted ×200), anti-CD68 antibody (mouse polyclonal, BMA Biomedicals, Switzerland; diluted
×100), anti-CD3 antibody (rabbit polyclonal, Abcam Plc., UK; diluted ×100), and anti-Nrf2
antibody (rabbit polyclonal, Abcam Plc., UK; diluted ×100) were used as the primary
antibodies and incubated overnight at 4°C. Each section was then incubated with EnVision
solution (Dako, Denmark) against mouse or rabbit immunoglobulin (Ig) G for 30 min at room
temperature. Antibody-binding was visualized using 3,3-diaminobenzidine (DAB) chromogen
and counterstained with Mayer’s hematoxylin. The number of each type of antibody-positive
cells was counted in 50 fields per section at 400× magnification in the outer segment of
the outer medulla in the right and left kidneys, and an average number per field was
calculated.
containing 0.3% hydrogen peroxide for 30 min to block endogenous peroxidase activity. The
sections were then treated with 10% normal goat serum for 30 min to block nonspecific
reactions. Anti-α-smooth muscle actin (α-SMA) antibody (mouse polyclonal, Dako, Denmark;
diluted ×200), anti-CD68 antibody (mouse polyclonal, BMA Biomedicals, Switzerland; diluted
×100), anti-CD3 antibody (rabbit polyclonal, Abcam Plc., UK; diluted ×100), and anti-Nrf2
antibody (rabbit polyclonal, Abcam Plc., UK; diluted ×100) were used as the primary
antibodies and incubated overnight at 4°C. Each section was then incubated with EnVision
solution (Dako, Denmark) against mouse or rabbit immunoglobulin (Ig) G for 30 min at room
temperature. Antibody-binding was visualized using 3,3-diaminobenzidine (DAB) chromogen
and counterstained with Mayer’s hematoxylin. The number of each type of antibody-positive
cells was counted in 50 fields per section at 400× magnification in the outer segment of
the outer medulla in the right and left kidneys, and an average number per field was
calculated.
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