The HPLC-DAD system (Hitachi, Tokyo, Japan) consisted of a pump (L-2130), autosampler (L-2200), column oven (L-2300) and UV/VIS diode array detector (L-2455). The output signal of the detector was recorded using EZChrom Elite software for Hitachi. For sample analysis, an OptimaPak C18 column (4.6 × 250 mm, 5 μm; RS Tech Co., Daejeon, Korea) was used, and the column oven temperature was kept at 35°C. The injection volume was 20 μL, and the flow rate of the mobile phase was 1.0 mL/min. The wavelength of the UV detector was set at 220 nm. The mobile phase was water containing 0.1% TFA and acetonitrile, with gradient elution at a flow rate of 1.0 mL/min (Table 3 ).
Hplc dad system
Manufactured by Hitachi
Sourced in Japan, Germany
The Hitachi HPLC-DAD system is a high-performance liquid chromatography (HPLC) instrument equipped with a diode-array detector (DAD). The system is designed to separate, identify, and quantify various compounds in complex mixtures. The HPLC component utilizes high-pressure liquid flow to facilitate the separation of analytes, while the DAD provides detailed spectral information for identification and quantification purposes.
Automatically generated - may contain errors
Lab products found in correlation
L 2130, by Hitachi (1 mentions)
L 2200, by Hitachi (1 mentions)
Ezchrom elite, by Hitachi (1 mentions)
L 2455, by Hitachi (1 mentions)
L 2300, by Hitachi (1 mentions)
Cyanidin 3 o glucoside, by Merck Group (1 mentions)
Gemini c18 column, by Phenomenex (1 mentions)
Ezchrome elite, by Hitachi (1 mentions)
3 protocols using hplc dad system
1
HPLC-DAD Analysis of Compounds
2
Quantification of Glucosinolates and Anthocyanins
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Glucosinolates were identified following their UV-Vis spectra, retention times, and their fragmentation patterns (M-and MSn) by HPLC-DAD-ESI-MSn, according to the method described by Baenas et al. (2016) (link). Chromatograms were recorded at 227 nm, and intact glucosinolates were quantified using glucoerucin and glucobrassicin as external standards for aliphatic and indolic glucosinolates, respectively (Sigma Aldrich, St. Louis, MO, USA).
The analysis was carried out in triplicate and the results were expressed as mg 100 g -1 DW.
For anthocyanin analysis, peak identification was also performed in the HPLC-DAD-ESI-MSn system using previously established conditions for these compounds in cruciferous sprouts (Baenas et al., 2015) (link). The extracted samples were quantified by a Hitachi HPLC-DAD system (Hitachi technologies, MERCK, Darmstadt, Germany) using the same chromatographic conditions, recording chromatograms at 520 nm, and using cyanidin 3-Oglucoside as external standard (Sigma-Aldrich, St. Louis, MO, USA). The analysis was carried out in triplicate and the results were expressed in mg 100 g -1 DW.
The analysis was carried out in triplicate and the results were expressed as mg 100 g -1 DW.
For anthocyanin analysis, peak identification was also performed in the HPLC-DAD-ESI-MSn system using previously established conditions for these compounds in cruciferous sprouts (Baenas et al., 2015) (link). The extracted samples were quantified by a Hitachi HPLC-DAD system (Hitachi technologies, MERCK, Darmstadt, Germany) using the same chromatographic conditions, recording chromatograms at 520 nm, and using cyanidin 3-Oglucoside as external standard (Sigma-Aldrich, St. Louis, MO, USA). The analysis was carried out in triplicate and the results were expressed in mg 100 g -1 DW.
3
HPLC-DAD Analysis of Bioactive Compounds
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The analysis was carried out using a Hitachi HPLC–DAD system equipped with a solvent delivery unit, autosampler, column oven, and diode-array detector. The acquired data were processed using EZChrome Elite for Hitachi. Separation was performed on a Gemini C18 column (4.6 mm × 250 mm, 5 μm; Phenomenex, Torrance, CA, USA) at 35°C. The mobile phase, consisting of solvent A (1% aqueous acetic acid, v/v) and solvent B (acetonitrile with 1% acetic acid, v/v), was eluted using the gradient procedure, which was as follows: 5-40% (B) over 0-30 min, 40-100% (B) over 30-40 min, held for 5 min, and then re-equilibrated to 5% for 15 min. The flow rate was 1.0 mL/min and the injection volume was set to 10 μL. The optimized detection wavelengths for standard compounds were set at 230, 272, and 280 nm.
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