Human lung cancer stem cell complete growth medium

Human lung ADC cell line SK-LU-1 and human lung SCC cell line NCI-H1703 were purchased from American Type Culture Collections (ATCC, Manassas, VA, USA). Human lung cancer stem cell (HLCSC) primary cell line was purchased from Celprogen (Torrance, CA, USA). SK-LU-1 and NCI-H1703 were routinely maintained in Gibco’s Dulbecco’s modified Eagle’s medium (DMEM) with 4.5 g/L d-glucose (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 1% (v/v) of 100 mM Gibco’s sodium pyruvate, 1% (v/v) of 10,000 U/mL Penicillin–10,000 μg/mL Streptomycin (Pen-Strep) and 10% (v/v) of Gibco’s fetal bovine serum (FBS). HLCSC was routinely maintained in human lung cancer stem cell complete growth medium with serum (Celprogen). All cell cultures were maintained in 5% CO₂ humidified atmospheric condition CO₂ incubator at 37 °C. Passaging was regularly performed with Sigma-Aldrich’s (St. Louis, MO, USA) 0.25% (w/v) trypsin–ethylenediaminetetraacetic acid (EDTA) solution.

Human lung squamous cell carcinoma cell line NCI-H1703 and human lung adenocarcinoma cell line SK-LU-1 were purchased from American Type Culture Collections (ATCC, Manassas, VA, USA). Human lung cancer stem cells (HLCSCs) primary cell line was purchased from Celprogen (Torrance, CA, USA). NCI-H1703 and SK-LU-1 were routinely maintained in Gibco’s Dulbecco’s modified Eagle’s medium (DMEM) with 4.5 g/L d-glucose (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 1% (v/v) of 100 mM Gibco’s sodium pyruvate, 1% (v/v) of 10,000 U/mL Penicillin-10,000 μg/mL Streptomycin (Pen-Strep), and 10% (v/v) of Gibco’s fetal bovine serum (FBS). HLCSC was routinely maintained in human lung cancer stem cell complete growth medium with serum (Celprogen). All cell cultures were maintained in 5% CO₂ humidified atmospheric condition CO₂ incubator at 37 °C. Passaging was performed regularly with Sigma-Aldrich’s (St. Louis, MO, USA) 0.25% (w/v) trypsin-Ethylenediaminetetraacetic acid (EDTA) solution.