Ab119378

To assess the protein levels, cell lysates were prepared using lysis buffer containing 20 mm Tris/HCl (pH 7.4), 1% NP‐40, 5 mm EDTA, 100 mm NaF, 2 mm Na₃VO₄, 10 mm Na₄P₂O₇, and protease inhibitor cocktail (#862209; ThermoFisher Scientific, Waltham, MA, USA). Whole‐cell lysates (10 μg) were subjected to SDS/PAGE and immunoblotted with specific antibodies. For immunoblotting, anti‐PPARγ (sc‐7273; Santa Cruz Biotech, Santa Cruz, CA, USA), anti‐C/EBPβ (sc‐7962; Santa Cruz Biotech), C/EBPα (sc‐61; Santa Cruz Biotech), anti‐β‐catenin (#610154; BD Transduction Laboratories, San Jose, CA, USA), anti‐γ‐tubulin (T6557; Sigma‐Aldrich), anti‐FLAG (F1804; Sigma‐Aldrich), phospho‐Y654 FGFR1 (ab59194; Abcam, Cambridge, MA, USA), FGFR1 (#9740; Cell Signaling Technology, Beverly, MA, USA), phospho Y653/Y654 FGFR1‐4 (AF3285; R&D Systems, Minneapolis, MN, USA), FGFR2 (ab109372; Abcam), FGFR3 (ab133644; Abcam), and FGFR4 (ab119378; Abcam) antibodies were used. The proteins were visualized using Clarity™ Western ECL Blotting Substrates (Bio‐Rad, Hercules, CA, USA) or ECL‐Prime (ThermoFisher Scientific) and ChemiDoc Imaging System (Bio‐Rad).