Nadph

Manufactured by Solarbio

Sourced in China

NADPH is a coenzyme that plays a crucial role in various cellular processes. It serves as an electron donor in anabolic reactions, particularly in the reduction of oxidized molecules. NADPH is essential for the biosynthesis of lipids, nucleic acids, and other metabolites, and is also involved in the maintenance of cellular redox balance.

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Lab products found in correlation

6 phosphate glucose, by Solarbio (2 mentions) 6 p glucose dehydrogenase, by Solarbio (2 mentions) Hank s balanced salt solution hbss, by Solarbio (2 mentions) Ethyl acetate, by Merck Group (2 mentions) Omeprazole, by LGC (1 mentions) Tolbutamide, by LGC (1 mentions) Reverse transcription kit, by Tsingke (1 mentions) Testosterone, by Solarbio (1 mentions) Dextromethorphan, by LGC (1 mentions) 4 hydroxytolbutamide, by LGC (1 mentions) 5 hydroxy omeprazole, by LGC (1 mentions) 6β hydroxytestosterone, by LGC (1 mentions) Cyp1a2, by Abcam (1 mentions) Dithiothreitol (dtt), by Solarbio (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Solarbio (1 mentions) Bovine serum albumin (bsa), by Solarbio (1 mentions) Edta na2, by Solarbio (1 mentions) Fast blue rr salt, by Merck Group (1 mentions) Deuterium oxide, by Maclin Biochemical Technology (1 mentions) Geraniol, by Maclin Biochemical Technology (1 mentions)

6 protocols using nadph

Investigating Cytochrome P450 Enzymes

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The following are used in the study: testosterone, paracetamol, phenacetin, and NADPH (Beijing Solarbio Science & Technology Co., Ltd. China); dextran, dextromethorphan, omeprazole, 5-hydroxy omeprazole, tolbutamide, 4-hydroxytolbutamide, and 6β-hydroxytestosterone (Toronto Research Chemicals); BCA protein Kit (Lianke Biotechnology Corporate Limited, No. a81911151); primer (synthesized by Tsingke Biotechnology Co., Ltd.); reverse transcription Kit (Tsingke Biotechnology Co., Ltd., No. 20190328); and CYP1A2, cyp2d1, CYP3A4, CYP3A1, CYP2C19, and CYP2C11 antibodies (Abcam Company).

Tang Y, & Li Y. (2022). Compound Shuganjieyu Capsule on CYP450 Enzyme Activity. BioMed Research International, 2022, 3228738.

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Chlorpyrifos Enzymatic Activity Assay

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Chlorpyrifos, a 98% technical product, was provided by Hubei Sharonda Co., Ltd. (Jingzhou, China). Triton X-100 was purchased from Chengdu Haobo Technology Co., Ltd. (Chengdu, China). DTT, PMSF, NADPH, and bovine serum albumin (BSA) were purchased from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China), as were EDTA-Na₂ and Sodium Dodecyl Sulfate (SDS).

Ruan Y., Liu X., Gong C., Zhang Y., Shen L., Ali H., Huang Y, & Wang X. (2021). Cloning and Functional Verification of CYP408A3 and CYP6CS3 Related to Chlorpyrifos Resistance in the Sogatella furcifera (Horváth) (Hemiptera: Delphacidae). Biology, 10(8), 795.

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Pesticide Metabolism Enzyme Assay

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Beta-cypermethrin (95.2%) was purchased from Tianjin Longdeng Chemical Co., Ltd. 7-ethoxycoumarin (7-EC), 7-hydroxycoumarin (7-HC), phenylmethylsulfonyl (PMSF), dithiothreitol (DTT), phenythiourea (PTU), α-naphthyl acetate (α-NA), β-naphthyl acetate (β-NA),1-chloro-2,4-dinitrobezene (CDNB), reduced glutathione (GSH), and fast blue RR salt and sodium dodecyl sulfate (SDS) were obtained from Sigma Chemical Co. NADPH was obtained from Solarbio Life Sciences Co., Ltd. The other chemicals were purchased from commercial suppliers.

You C., Li Z., Yin Y., Na N, & Gao X. (2022). Time of Day-Specific Changes in Metabolic Detoxification and Insecticide Tolerance in the House Fly, Musca domestica L.. Frontiers in Physiology, 12, 803682.

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Chemoenzymatic Synthesis of (Z/E)-3''-OH-GHQ

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(Z/E)-3’’-hydroxy-geranylhydroquinone [(Z/E)-3’’-OH-GHQ] was chemically synthesized as described previously (Oshikiri et al., 2020 (link)). Cofactors FMN, NAD(P)⁺ and NAD(P)H were purchased from Solarbio (Beijing, China). Deuterium oxide and the substrates cinnamyl alcohol, p-coumaryl alcohol, coniferyl alcohol, sinapyl alcohol, and geraniol were purchased from Macklin (Shanghai, China).

Wang R., Liu C., Lyu C., Sun J., Kang C., Ma Y., Wan X., Guo J., Shi L., Wang J., Huang L., Wang S, & Guo L. (2023). The discovery and characterization of AeHGO in the branching route from shikonin biosynthesis to shikonofuran in Arnebia euchroma. Frontiers in Plant Science, 14, 1160571.

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Evaluation of Sulfonylurea Drugs

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Glimepiride (purity 99.8%), gliclazide (purity 99.9%), and gliquidone (purity 99.3%) were supplied by the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Fetal bovine serum (FBS) and Hank’s Balanced Salt Solution (HBSS) was from Solarbio Co., Ltd (Beijing, China). Dulbecco’s modified Eagle’s Medium (DMEM) was from Solarbio Co., Ltd. Methanol and ethyl acetate were from Merck Co. Ltd (Darmstadt, Germany). NADPH, 6-phosphate- glucose, and 6-p-glucose dehydrogenase were purchased from Solarbio Co., Ltd. Human CYP2C9*1, *2 and *3 recombinases (1 nmol/ml P450, 10 mg/ml protein) were provided by the Research Institute for Liver Diseases (Shanghai Corporation Limited, Shanghai, China). All other chemicals and solvents were of the highest grade or analytical grade commercially available.

Yang F., Xiong X., Liu Y., Zhang H., Huang S., Xiong Y., Hu X, & Xia C. (2018). CYP2C9 and OATP1B1 genetic polymorphisms affect the metabolism and transport of glimepiride and gliclazide. Scientific Reports, 8, 10994.

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Pharmacokinetics of Antidiabetic Drugs

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Glibenclamide (purity 99.9%), glipizide (purity 99.8%), and gliquidone (purity 99.3%) were supplied by the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS) and Hank’s balanced salt solution (HBSS) were provided by Solarbio Co., Ltd (Beijing, China). NADPH, 6-phosphate-glucose, and 6-p-glucose dehydrogenase were purchased from Solarbio Co., Ltd. Human CYP2C9*1, *2 and *3 recombinases (1nmol/ml P450, 10 mg/ml protein) were provided by the Research Institute for Liver Diseases (Shanghai Corporation Limited, Shanghai, China). Methanol and ethyl acetate were from Merck Co. Ltd (Darmstadt, Germany). All other chemicals and solvents were of the highest grade or analytical grade commercially available.

Yang F., Liu L., Chen L., Liu M., Liu F., Xiong Y., Hu X, & Xia C. (2018). OATP1B3 (699G>A) and CYP2C9*2, *3 significantly influenced the transport and metabolism of glibenclamide and glipizide. Scientific Reports, 8, 18063.

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