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α naphthylisothiocyanate

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α-naphthylisothiocyanate is a laboratory reagent used in chemical synthesis and analysis. It serves as a precursor for the production of various organic compounds. The compound is composed of a naphthyl group bonded to an isothiocyanate functional group. Its core function is to facilitate chemical reactions and labeling applications in a laboratory setting.

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Lab products found in correlation

Hydroxypropyl methylcellulose, by Merck Group (1 mentions) Compound 48 80, by Merck Group (1 mentions) Pentobarbital sodium, by Merck Group (1 mentions) Tauroursodeoxycholic acid, by Merck Group (1 mentions) Cholic acid, by Merck Group (1 mentions) Taurodeoxycholic acid, by Merck Group (1 mentions) Trizol solution, by Thermo Fisher Scientific (1 mentions) Phospho jnk p jnk, by Cell Signaling Technology (1 mentions) Lightcycle 480 sybr green 1 master mix, by Roche (1 mentions) ω muricholic acid, by Merck Group (1 mentions) Gapdh, by Abcam (1 mentions) Acetonitrile of high performance liquid chromatography, by Merck Group (1 mentions) Milli q system, by Merck Group (1 mentions) Antibody against gapdh, by Abcam (1 mentions) Phospho stat3 p stat3, by Cell Signaling Technology (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Chlorpromazine, by Merck Group (1 mentions) Methyltestosterone, by Merck Group (1 mentions) Ethinyl estradiol, by Merck Group (1 mentions) Nitrosodiethylamine, by Merck Group (1 mentions)

6 protocols using α naphthylisothiocyanate

1

Compound 48/80 and ANIT-Induced Liver Injury

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Compound 48/80 (Sigma) was dissolved in 0.9% NaCl to a concentration of 140 mg/mL. Mice received a single intradermal injection of 50µL 0.9% NaCl or Compound 48/80 solution. ANIT (α-naphthylisothiocyanate; Sigma) was dissolved in corn oil to a concentration of 12.5 mg/mL. Mice received oral gavage of 100 µL (50 mg/kg) per day for five consecutive days. The ATX-inhibitor PAT-04818 (link) was kindly donated by PharmAkea Therapeutics. It was dosed once daily by oral gavage (20 mg/kg/day) in a vehicle of 0.5% hydroxypropyl methylcellulose (Sigma-Aldrich 64,620) (3.3 mg/mL). Mice were injected intraperitoneally with 100 µL (40 mg/kg) per day for five consecutive days.
Langedijk J., Bolier R., Tolenaars D., ten Bloemendaal L., Duijst S., de Waart D., Beuers U., Bosma P, & Elferink R.O. (2021). Reduced spontaneous itch in mouse models of cholestasis. Scientific Reports, 11, 6127.
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2

Evaluation of Chinese Botanical Drugs

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All the Chinese botanical drugs used in this experiment were purchased from Beijing Tongrentang Co., Ltd. (Beijing, China). Pentobarbital sodium, UDCA, and α-naphthylisothiocyanate (ANIT) were purchased from Sigma-Aldrich Co. (St. Louis, MO, United States). Olive oil was chosen from Shanghai Yi En Chemical Technology Co., Ltd. (Shanghai, China). The chemical reagents used in this experiment were all of high-performance liquid chromatography (HPLC) analytical grade.
Han J., Wu P., Wen Y., Liu C., Liu X., Tao H., Zhang F., Zhang X., Ye Q., Shen T., Chen X, & Yu H. (2023). The zhuyu pill relieves rat cholestasis by regulating the mRNA expression of lipid and bile metabolism associated genes. Frontiers in Pharmacology, 14, 1280864.
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3

Experimental Cholestatic Liver Injury Assay

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α-Naphthylisothiocyanate (ANIT), cholic acid (CA), tauroursodeoxycholic acid (TUDCA), taurodeoxycholic acid (TDCA), tauro-ω-muricholic acid (TωMCA), tauro-α-muricholic acid (TαMCA), taurocholic acid (TCA), β-muricholic acid (βMCA) and ω-muricholic acid (ωMCA) were purchased from Sigma-Aldrich (St Louis, MO). Assay kits for the liver injury markers aspartate aminotransferase (AST), alanine aminotransferase (ALT), cholestasis markers total bile acid (TBA), alkaline phosphatase (ALP), direct bilirubin (DBIL), and total bilirubin (TBIL) were purchased from Ruiyuan Biotechnology (Ningbo, China). TRIzol solution was purchased from Invitrogen (Dalian, China). The reverse transcription kit and LightCycle 480 SYBR Green I Master Mix were purchased from Roche Diagnostics (Shanghai, China). Antibodies against total mitogen-activated protein kinase kinase 4 (t-MKK4), phospho-mitogen-activated protein kinase kinase 4 (p-MKK4), total-JNK (t-JNK), and phospho-JNK (p-JNK) were obtained from Cell Signaling Technology (Danvers, MA). Antibodies against NF-κB subunit p65, phospho-p65 (p-p65), total STAT3 (t-STAT3), phospho-STAT3 (p-STAT3), and GAPDH were acquired from Abcam.
Dai M., Hua H., Lin H., Xu G., Hu X., Li F., Gonzalez F.J., Liu A, & Yang J. (2018). Targeted Metabolomics Reveals a Protective Role for Basal PPARα in Cholestasis Induced by α-Naphthylisothiocyanate. Journal of proteome research, 17(4), 1500-1508.
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4

Extraction and Preparation of Ginger Extract

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Acetonitrile of high performance liquid chromatography was purchased from Merck (Darmstadt, Germany). Ultrapure water was further purified using a Milli-Q system (Millipore, Bedford, MA, USA). α-naphthylisothiocyanate (ANIT) was purchased from Sigma-Aldrich (MO, USA). Zingiber officinale Rosc. was purchased from Harbin Tong Ren Tang Drug Store (Harbin, China). Alcohol was purchased from Beijing Reagent Company (Beijing, China). Scoparone (purity 99%) was purchased from Sichuan Provincial Institute for Food and Drug Control (Sichuan, P. R. China). ELISA kit for UDP-glucuronosyltransferase 1A1 (Shanghai Shuangying Biological Technology Co.,Ltd, China).
Zingiber officinale Rosc. (50.8 g) was decocted in 500 mL distilled water for 1 h and then filtered through 6 layers of gauze. The residue was added into 10 times the volume of distilled water for being decocted for 1 h. The process was repeated 3 times before combining three filtrates for furthering concentration to 500 mL. 15.6 mL was added to 104.4 mL of distilled water to prepare the orally administered solution at the concentration of 0.013 g/mL.
Fang H., Zhang A., Yu J., Wang L., Liu C., Zhou X., Sun H., Song Q, & Wang X. (2016). Insight into the metabolic mechanism of scoparone on biomarkers for inhibiting Yanghuang syndrome. Scientific Reports, 6, 37519.
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5

ANIT-Induced Liver Injury Model

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Chlorogenic acid (98%, Figure 1a) was obtained from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). α-Naphthyl isothiocyanate (ANIT, 95%) was purchased from Sigma Aldrich (Louis, MO, USA). Trizol was provided by Invitrogen (Carlsbad, CA, USA). Alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bile acid (TBA), direct bilirubin (DBIL) and indirect bilirubin (IBIL) assay kits were from Yonghe Sunshine Technology (Changsha, China). Antibodies against NFκB subunit p65 and active form p-p65, total STAT3 (t-STAT3) and phospho-STAT3 (p-STAT3) were purchased from Cell Signaling (Danvers, MA, USA). Antibody against GAPDH was purchased from Abcam (Cambridge, MA, USA). Ultrapure water was freshly prepared using a Milli-Q50 SP Reagent Water System (Bedford, MA, USA). All the other chemicals were of the highest grade from commercial sources.
Tan Z., Luo M., Yang J., Cheng Y., Huang J., Lu C., Song D., Ye M., Dai M., Gonzalez F.J., Liu A, & Guo B. (2016). Chlorogenic acid inhibits cholestatic liver injury induced by α-naphthylisothiocyanate: involvement of STAT3 and NFκB signalling regulation. The Journal of pharmacy and pharmacology, 68(9), 1203-1213.
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6

Liver Tissue Characterization Protocol

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Chemicals. Acetaminophen (AP), α-naphthylisothiocyanate (ANIT), chloramphenicol (CH), chlorpromazine (CP), colchicine (CL), cyclosporine (CS), ethinyl estradiol (EE), methyl testosterone (MT), and nitroso diethylamine (ND) were purchased from Sigma-Aldrich (St.Louis, MO, USA). Benziodarone (BD) was purchased from Kemprotec Limited (Middlesbrough, United Kingdom). Stock solutions were prepared for all the compounds in DMSO (VWR, Briare, France) except for APAP. For APAP, stock solutions were prepared using the WME medium. RNAlater reagent was purchased from Sigma-Aldrich (St.Louis, MO, USA).
Human Liver Tissue. Human tissue was obtained from the remaining liver tissue after split liver transplantation (TX) from six donors. The characteristics of the human livers used in the experiments are described in Table 1. The experimental protocols were approved by the Medical Ethical Committee of the University Medical Center Groningen.
Vatakuti S., Pennings J.L., Gore E., Olinga P, & Groothuis G.M. (2016). Classification of Cholestatic and Necrotic Hepatotoxicants Using Transcriptomics on Human Precision-Cut Liver Slices. Chemical research in toxicology, 29(3).
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