Mouse spleen cells were isolated from Parp9+/+ (WT) and Parp9-/- (KO) mice infected with VSV for 1 day. The cells were then fixed and stained with Zombie Aqua fixable viability kit (423102, Biolegend), APC/Cyanine7 anti-mouse CD45 antibody (30-F11, Biolegend), FITC anti-mouse CD3 antibody (17A2, Biolegend), PE/Cyanine7 anti-mouse CD4 antibody (RM4-5, Biolegend), PerCP/Cyanine5.5 anti-mouse CD8a antibody (53-6.7, Biolegend) and APC anti-mouse CD19 antibody (1D3/CD19, Biolegend) (1 μl antibody for 2 million cells) for analyzing composition of CD4+, CD8+ and B cells. Flow cytometry data were acquired on a LSR-II flow cytometer (Beckton Dickinson) and analyzed using FlowJo v10 software (Tree Start)34 (link).
Apc cyanine7 anti mouse cd45 antibody 30 f11
Manufactured by BioLegend
The APC/Cyanine7 anti-mouse CD45 antibody (30-F11) is a fluorophore-conjugated monoclonal antibody that binds to the CD45 protein expressed on the surface of mouse immune cells. It can be used for the identification and characterization of various mouse hematopoietic cell types in flow cytometry applications.
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Lab products found in correlation
Zombie aqua fixable viability kit, by BioLegend (3 mentions)
Lsrii flow cytometer, by BD (3 mentions)
Pe anti mouse cd324 e cadherin antibody decma 1, by BioLegend (2 mentions)
Pe cyanine7 anti mouse cd326 epcam antibody g8, by BioLegend (2 mentions)
Fitc anti mouse cd3 antibody 17a2, by BioLegend (1 mentions)
3 protocols using apc cyanine7 anti mouse cd45 antibody 30 f11
1
Immune cell composition in VSV-infected mice
2
Isolation and Flow Cytometry of Intestinal Epithelial Cells
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Mouse primary IECs were isolated from wild-type
Dhx15fl/fl and
Dhx15IEC-KO mice. The
cells were then fixed and stained with APC/Cyanine7 anti-mouse CD45 antibody
(30-F11, Biolegend), PE anti-mouse CD324 (E-Cadherin) antibody (DECMA-1,
Biolegend), PE/Cyanine7 anti-mouse CD326 (EpCAM) antibody (G8.8, Biolegend)
and their isotype matched control antibodies for their differentiation.
Cells isolated from spleen, mesenteric lymph node (LN), and lamina propria
lymphocytes were stained using live/dead Zombie Aqua Fixable viability Kit
(Biolegend) for 10 minutes followed by staining with fluorochrome-conjugated
antibodies on ice for 20 minutes, washed twice in PBS/BSA, and fixed in 1%
paraformaldehyde prior to flow cytometry analysis. Flow cytometry data were
acquired on a LSR-II flow cytometer (Beckton Dickinson) and analyzed using
FlowJo v10 software (Tree Start) as previously described (Xing et al., 2016 (link)).
Dhx15fl/fl and
Dhx15IEC-KO mice. The
cells were then fixed and stained with APC/Cyanine7 anti-mouse CD45 antibody
(30-F11, Biolegend), PE anti-mouse CD324 (E-Cadherin) antibody (DECMA-1,
Biolegend), PE/Cyanine7 anti-mouse CD326 (EpCAM) antibody (G8.8, Biolegend)
and their isotype matched control antibodies for their differentiation.
Cells isolated from spleen, mesenteric lymph node (LN), and lamina propria
lymphocytes were stained using live/dead Zombie Aqua Fixable viability Kit
(Biolegend) for 10 minutes followed by staining with fluorochrome-conjugated
antibodies on ice for 20 minutes, washed twice in PBS/BSA, and fixed in 1%
paraformaldehyde prior to flow cytometry analysis. Flow cytometry data were
acquired on a LSR-II flow cytometer (Beckton Dickinson) and analyzed using
FlowJo v10 software (Tree Start) as previously described (Xing et al., 2016 (link)).
3
Isolation and Flow Cytometry of Intestinal Epithelial Cells
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