A20 b cell lymphoma cell line

The 4T1 BC cell line and the A20 B cell lymphoma cell line were purchased from ATCC, (Manassas, VA, USA), expanded and stored according to the producer’s instructions. Cells were tested and authenticated by the StemElite ID System (Promega, Fitchburg, WI, USA). Cells were tested every six months for Mycoplasma by means of the ATCC Universal Mycoplasma Detection Kit 30–1012, cultured for no more than two weeks and used for no longer than 15 passages.

The CT26 colorectal carcinoma cell line (derived from BALB/c mice) (ATCC, Cat#CRL-2638™), B16-F10 skin melanoma cell line (derived from C57BL/6J mice) (ATCC, Cat#CRL-6475™), A20 B cell lymphoma cell line (derived from BALB/cAnN mice) (ATCC, Cat#TIB-208™), and 4T1, an animal stage IV breast cancer cell line (derived from BALB/cfC3H mice) (ATCC, Cat#CRL-2539™) were purchased from ATCC. The Huh-7 human liver cell line was obtained from Creative Biolabs. The 293T human embryonic kidney cell line was purchased from ATCC (ATCC, Cat#CRL-11268™). The CT26, A20, 4T1, and Huh-7 cell lines were maintained in RPMI1640 medium containing 10% FBS, 100 units/mL penicillin/streptomycin, 2 mM l-glutamine, 50 µg/mL Normocin, and 2.0 g/L sodium bicarbonate. The B16-F10 and 293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS), 100 units/mL penicillin/streptomycin, 4 mM l-glutamine, 4.5 g/L glucose, 1 mM sodium pyruvate, and 1.5 g/L sodium bicarbonate. All cell lines were cultivated in a humidified atmosphere containing 5% CO₂ at 37 °C. The use of the human cell line for xenografts in mice was approved by the Animal Care and Use Committees (ACUC) and registered with the Institutional Biosafety Committee (IBC) at NCI-Frederick, NIH.