G3000pwxl column

The molecular weight distribution of MW hydrolysates was obtained by Size Exclusion Chromatography (SEC) using High-Performance Liquid Chromatography equipped with a TSKgel G3000PWxl column (7.8 mm I.D × 30 cm, 7 µm) and a DAD detector (Agilent infinity 1260 II LC system, Santa Clara, CA, USA). Briefly, 10 mg/mL of each MW hydrolysate solution and 1 mg/mL of the standard (1–670 kDa) mixture were eluted using a phosphate buffer (pH 7.4) at a flow rate of 0.5 mL/min and were detected at 220 nm. Calibration curves were constructed using Throglobulin (670 kDa), γ-globulin (150 kDa, Ovalbumin (45 kDa), Myoglobin (17 kDa), Aprotinin (6.7 kDa), and Angiotensin II (1 kDa) as the protein standards. The peptide molecular mass distribution was estimated from a logarithmic calibration curve that was constructed by plotting the elution time (in min) against MW (in kDa) (Figure S1) and the peak area to estimate the proportion of each molecular weight of the peptides present.

The high-performance liquid chromatography (HPLC) measurement of PZMP3-1’s Mw was detected on an Agilent instrument (LC 1200, USA) with a 7.8 × 300 mm TSK-gel G3000PWxl column. Using a calibration curve, the Mw concerning dextran was calculated (26 (link)).
Utilizing a collection of monosaccharides as a standard, the Shimadzu GC (2014 C) with a high-performance capillary column DB-17 (30 ml × 0.25 mm ID; 0.25 μm film thickness, Agilent) measured the various monosaccharide by comparing retention durations and peak regions (27 (link)).