Dmrxa2 fluorescence microscope

Manufactured by Leica

Sourced in Germany

The DMRXA2 is a fluorescence microscope designed for advanced imaging and analysis. It features a high-intensity illumination system, improved optical components, and a modular design to accommodate a variety of sample types and experimental requirements. The DMRXA2 supports multiple fluorescence techniques, providing researchers with a versatile tool for their scientific investigations.

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Lab products found in correlation

Texas red avidin dcs, by Vector Laboratories (1 mentions) Biotinylated anti avidin d, by Vector Laboratories (1 mentions) Biotin 14 dutp, by Thermo Fisher Scientific (1 mentions) Volocity, by PerkinElmer (1 mentions) Chicken serum, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Naphtol as b1 phospate, by Merck Group (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Texas red, by Merck Group (1 mentions) Xt microscope control software, by Thermo Fisher Scientific (1 mentions) Quanta 3d feg dual beam fib sem, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Boster Bio (1 mentions) γh2ax antibody, by Merck Group (1 mentions) Dfc350 fx camera, by Leica (1 mentions) Dfc300fx camera, by Leica (1 mentions) Prism 4, by GraphPad (1 mentions)

Close spelling variants of this product

DMRXA2 microscope DMRXA2 Fluorescence microscope

6 protocols using dmrxa2 fluorescence microscope

Placental Vasculature Quantification

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Fluorescence micrographs were acquired using a Leica DMRXA2 fluorescence microscope equipped with a Leica DFC350FX camera. Histochemical micrographs were acquired using a Leica DMRXA2 fluorescence microscope equipped with a Leica DFC300FX camera. Images were processed using Adobe Photoshop.
Blood vessels in placenta labyrinth were counted as previously described [45] (link). Statistical analysis was conducted using unpaired t-test by GraphPad Prism 4. Results with p values less than 0.05 were considered significant.

Du X., Dong Y., Shi H., Li J., Kong S., Shi D., Sun L.V., Xu T., Deng K, & Tao W. (2014). Mst1 and Mst2 Are Essential Regulators of Trophoblast Differentiation and Placenta Morphogenesis. PLoS ONE, 9(3), e90701.

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Chromosome Preparation and In Situ Hybridization

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Chromosome preparations and in situ hybridization were performed as described in D’Hont et al. (2000) with modifications. BAC clones (Table S3) from accession DH‐Pahang (D’Hont et al., 2012; http://banana‐genome.cirad.fr) were labeled by random priming with biotin‐14‐dUTP (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA) or Alexa 488‐5‐dUTP (Life Technologies; Thermo Fisher Scientific). Chromosome preparations were incubated in RNAse A (100 ng µl^–1) and pepsin (100 mg ml^–1) in 0.01 m HCl and fixed with paraformaldehyde (4%). Biotinylated probes were immunodetected by Texas Red avidin DCS (Vector Laboratories, Burlingame, CA, USA) and the signal was amplified with biotinylated antiavidin D (Vector Laboratories). Fluorescence images were captured using a cooled high‐resolution black and white CCD camera (ORCA; Hamamatsu, Hamamatsu City, Japan) fitted on a DMRXA2 fluorescence microscope (Leica Microsystems, Wetzlar, Germany) and analysed using volocity (Perkin Elmer, Waltham, MA, USA).

Martin G., Baurens F., Hervouet C., Salmon F., Delos J., Labadie K., Perdereau A., Mournet P., Blois L., Dupouy M., Carreel F., Ricci S., Lemainque A., Yahiaoui N, & D’Hont A. (2020). Chromosome reciprocal translocations have accompanied subspecies evolution in bananas. The Plant Journal, 104(6), 1698-1711.

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Characterization of Pluripotency and Differentiation Markers

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The ES R1 and EC F9 cells were fixed with 2% paraformaldehyde in phosphate-buffered saline, PBS, pH 7.0, within 15 min. ALP activity was detected after incubation in a solution containing 10 ml 0.02 M Tris-HCl buffer (pH 8.7), 1 mg Naphtol-AS-B1-phospate, and 5 mg fast red dye Texas Red (all from Sigma) at 37°C for 1 h.
For immunofluorescence analysis, cells fixed in 4% paraformaldehyde in PBS for 1 h were washed and permeabilized with 0.5% Triton X-100. Nonspecific reactions were blocked by 10% chicken serum (Gibco/Invitrogen). Primary rabbit anti-Oct4 and goat anti-Gata4 antibodies (Santa Cruz Biotechnology) were used at a dilution of 1 : 100. The cells were incubated in a solution of primary antibodies in PBS-Tween 20 at 4°C overnight. Secondary chicken anti-rabbit and donkey anti-goat antibodies conjugated with Alexa Fluor 594 and Alexa Fluor 488 (Molecular Probes) were diluted at 1 : 800 in a blocking buffer and applied to the cells for 4 h at room temperature. DAPI (Molecular Probes) was applied for 15 min for nuclear staining. The cells were mounted and examined under a Leica DMRXA2 fluorescence microscope (Leica Microsystems GmbH). For negative controls, the primary antibodies were omitted, and the same staining procedure was used.

Gordeeva O, & Khaydukov S. (2017). Tumorigenic and Differentiation Potentials of Embryonic Stem Cells Depend on TGFβ Family Signaling: Lessons from Teratocarcinoma Cells Stimulated to Differentiate with Retinoic Acid. Stem Cells International, 2017, 7284872.

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Characterization of NMBs on Glass Slides

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A Pro-4 four point system (Lucas Labs, Gilroy, CA, USA) was used in resistivity measurements. The system was made up of a Keithley 2400, a Pro4, and a notebook with the Pro4 V1.2.4 software installed. The system uses the dual configuration test method of ASTM standard F84-99 to compensate for errors in probe spacing and errors caused by proximity to the edge of the conducting layer. A V/I measurement was taken and recorded and the subsequent resistivity was computed.
Scanning electron microscope (SEM) micrographs were obtained using a Quanta 3D FEG Dual Beam FIB/SEM and its xT Microscope Control Software (FEI, Hillsboro, OR, USA). Fluorescence studies were performed on samples using a DM RXA2 fluorescence microscope (Leica, Wetzlar, Germany) fitted with a 100× objective lens with a 1.4 N.A. Slidebook version 4.1 was used for image acquisition from a SensiCam QE camera (Cooke, Auburn Hills, MI, USA) attached to the microscope.
Optical images were obtained from a 40 CFL inverted microscope (Axiovert, Thornwood, NY, USA) fitted with a 20× objective with a 1.0 N.A. AxioVision 4.6.1.0 was used for image acquisition from an AxioCam MRc camera attached to the microscope. Percentage area covered by the NMBs on the glass slides were determined using the ImageJ 1.46r software (Wayne Rasband, National Institutes of Health, USA).

Gikunoo E., Abera A, & Woldesenbet E. (2014). A Novel Carbon Nanofibers Grown on Glass Microballoons Immunosensor: A Tool for Early Diagnosis of Malaria. Sensors (Basel, Switzerland), 14(8), 14686-14699.

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Immunofluorescence Staining of γH2AX

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The cells covered 80–90% of the glass coverslips. The cells were washed three times with PBS and fixed in 4% paraformaldehyde (Boster, Wuhan, China). Then, the cells were permeabilized with 0.1% Triton X-100 and blocked in 4% bovine serum albumin. Images were obtained using a DM-RXA2 fluorescence microscope (Leica) after incubation with γH2AX antibody (Merck Millipore, Darmstadt, Germany), a fluorescence-conjugated secondary antibody, and DAPI (4′,6-diamidino-2-phenylindole) stain (Helixgen, Guangzhou, China).

Tian X., Liu C., Wang X., Wang F., Wang L., Xu L., Ma J., Gao Y., Bao Y., Wang F., Sun L., Wei J., Lin C., Zhang H., Zhu G., Guan X., Fu S, & Zhang C. (2018). Sei-1 promotes double minute chromosomes formation through activation of the PI3K/Akt/BRCA1-Abraxas pathway and induces double-strand breaks in NIH-3T3 fibroblasts. Cell Death & Disease, 9(3), 341.

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Visualizing Actin and Cell Nuclei

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Following 18 and 72 hours of culture, cells were washed with PBS and fixed using a solution of 3.7% paraformaldehyde for 10 min. The fixed cells were washed again with PBS and permeabilised with 0.5% Triton X-100 for 10 min. Tetramethylrhodamine isothiocyanate conjugated phalloidin (Pha-TRITC) 0.2 μg mL -1 (Sigma, St. Louis, MO, USA) was used to visualise the actin cytoskeleton. Additionally, the top surface of the discs was coated with Fluoroshield antifade mountant containing 4',6-diamidino-2-phenylindole (DAPI) (Vector Laboratories) to stain cell nuclei. For the 18-hour time point wheat germ agglutinin-AlexaFluor488 conjugate was used at a concentration of 5 µg mL -1 to stain the cell membranes according to manufacturer's instructions. Samples were examined using a Leica DMRXA2 fluorescence microscope.

Tanase C.E., Golozar M., Best S.M, & Brooks R.A. (2019). Cell response to plasma electrolytic oxidation surface-modified low-modulus β-type titanium alloys. Colloids and surfaces. B, Biointerfaces, 176.

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