Blood vessels in placenta labyrinth were counted as previously described [45] (link). Statistical analysis was conducted using unpaired t-test by GraphPad Prism 4. Results with p values less than 0.05 were considered significant.
Dmrxa2 fluorescence microscope
The DMRXA2 is a fluorescence microscope designed for advanced imaging and analysis. It features a high-intensity illumination system, improved optical components, and a modular design to accommodate a variety of sample types and experimental requirements. The DMRXA2 supports multiple fluorescence techniques, providing researchers with a versatile tool for their scientific investigations.
Lab products found in correlation
6 protocols using dmrxa2 fluorescence microscope
Placental Vasculature Quantification
Blood vessels in placenta labyrinth were counted as previously described [45] (link). Statistical analysis was conducted using unpaired t-test by GraphPad Prism 4. Results with p values less than 0.05 were considered significant.
Chromosome Preparation and In Situ Hybridization
Characterization of Pluripotency and Differentiation Markers
For immunofluorescence analysis, cells fixed in 4% paraformaldehyde in PBS for 1 h were washed and permeabilized with 0.5% Triton X-100. Nonspecific reactions were blocked by 10% chicken serum (Gibco/Invitrogen). Primary rabbit anti-Oct4 and goat anti-Gata4 antibodies (Santa Cruz Biotechnology) were used at a dilution of 1 : 100. The cells were incubated in a solution of primary antibodies in PBS-Tween 20 at 4°C overnight. Secondary chicken anti-rabbit and donkey anti-goat antibodies conjugated with Alexa Fluor 594 and Alexa Fluor 488 (Molecular Probes) were diluted at 1 : 800 in a blocking buffer and applied to the cells for 4 h at room temperature. DAPI (Molecular Probes) was applied for 15 min for nuclear staining. The cells were mounted and examined under a Leica DMRXA2 fluorescence microscope (Leica Microsystems GmbH). For negative controls, the primary antibodies were omitted, and the same staining procedure was used.
Characterization of NMBs on Glass Slides
Scanning electron microscope (SEM) micrographs were obtained using a Quanta 3D FEG Dual Beam FIB/SEM and its xT Microscope Control Software (FEI, Hillsboro, OR, USA). Fluorescence studies were performed on samples using a DM RXA2 fluorescence microscope (Leica, Wetzlar, Germany) fitted with a 100× objective lens with a 1.4 N.A. Slidebook version 4.1 was used for image acquisition from a SensiCam QE camera (Cooke, Auburn Hills, MI, USA) attached to the microscope.
Optical images were obtained from a 40 CFL inverted microscope (Axiovert, Thornwood, NY, USA) fitted with a 20× objective with a 1.0 N.A. AxioVision 4.6.1.0 was used for image acquisition from an AxioCam MRc camera attached to the microscope. Percentage area covered by the NMBs on the glass slides were determined using the ImageJ 1.46r software (Wayne Rasband, National Institutes of Health, USA).
Immunofluorescence Staining of γH2AX
Visualizing Actin and Cell Nuclei
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