Dulbecco s phosphate buffered saline dpbs 10

Sodium alginate was purchased from ACROS Organics^TM (Thermo Fisher Scientific, Waltham, MA, USA). Poly(vinyl alcohol); quercetin (2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4Hchromen-4-one); 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS); potassium persulfate; 2,2-diphenyl-1-picrylhydrazyl radical (DPPH); methanol; L-α-phosphatidylcholine (EPC); dimethylsulfoxide (DMSO); Dulbecco’s phosphate buffered saline (DPBS) (10×); ethanol (absolute); sodium cholesteryl sulphate; trypsin (from porcine pancreas); and the Millicell Cell Culture Inserts (12 mm, polycarbonate, 0.4 µm) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cholesterol (ovine wool) and egg chicken ceramide and were obtained from Avanti Polar Lipids (Alabaster, AP, USA). The porcine ears were acquired in a local slaughterhouse located in Porto, Portugal. The ultra-pure water system (Arium Pro, Sartorius AG, Gottingen, Germany) was the source of double-deionized water. Reagents were weighted in a Kern ACJ/ACS 80-4 (Kern & Sohn; Balingen, Germany) digital analytical balance, and a Crison pH meter GLP 22 with a Crison 52-02 tip (Crison; Barcelona, Spain) was used to perform the necessary pH measurements.

The mammary pheromone (MP), i.e., 2-methylbut-2-enal (2MB2; CAS # 497-03-0) and the 2-methylpent-2-enal (2MP2, CAS # 623-36-9), L-glutathione reduced (CAS # 70-18-8), mucin from bovine submaxillary glands (bovine mucin, CAS # 84195-52-8) and sterile filtered Dulbecco’s Phosphate Buffered Saline (DPBS 10×) were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France).