Anti n cadherin rabbit monoclonal antibody

Manufactured by Cell Signaling Technology

Sourced in United States

The Anti-N-cadherin rabbit monoclonal antibody is a laboratory reagent designed for the detection and analysis of N-cadherin protein expression. N-cadherin is a cell-cell adhesion molecule that plays a role in cell-cell interactions and signaling. This antibody can be used in various immunoassay techniques to identify and quantify N-cadherin in biological samples.

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Lab products found in correlation

Anti e cadherin rabbit monoclonal antibody, by Cell Signaling Technology (3 mentions) Anti vimentin rabbit monoclonal antibody, by Cell Signaling Technology (2 mentions) Polyvinylidene fluoride membranes, by Cell Signaling Technology (1 mentions) Polyvinylidene fluoride membrane, by GE Healthcare (1 mentions) Anti α sma rabbit polyclonal antibody, by Abcam (1 mentions) Anti collagen 1 rabbit polyclonal antibody, by Abcam (1 mentions) Anti fibronectin mouse monoclonal antibody, by BD (1 mentions) Anti phospho smad2 antibody, by Cell Signaling Technology (1 mentions) Rabbit anti β actin monoclonal antibody, by Cell Signaling Technology (1 mentions) Anti human β actin antibody, by Abcam (1 mentions)

Close spelling variants of this product

Rabbit monoclonal anti-N-cadherin Rabbit anti-N-cadherin antibody Monoclonal anti-N-cadherin Rabbit anti-N-cadherin Anti-N-cadherin antibody N-cadherin antibody Anti-N-cadherin Monoclonal rabbit antibody N-cadherin Monoclonal rabbit

3 protocols using anti n cadherin rabbit monoclonal antibody

Western Blot Analysis of Cellular Signaling

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Cells were lysed with Nonidet P-40 (NP-40) lysis buffer (50 mM Tris–HCl [pH 8.0], 150 mM NaCl, 1% NP-40) containing protease inhibitor cocktail. Proteins were separated on sodium dodecyl sulfate‑polyacrylamide gel electrophoresis gels and were electroblotted onto polyvinylidene fluoride membrane (GE Healthcare Bio-sciences, Piscataway, NJ). The polyvinylidene fluoride membranes were incubated with anti-phospho-Smad2 antibody (Ser465/467), anti-phospho-extracellular-signal-regulated kinases (ERK) 1/2 monoclonal antibody (Thr202/Tyr204), anti-total-Smad2 mouse monoclonal antibody, anti-total-ERK1/2 rabbit monoclonal antibody, anti-E-cadherin rabbit monoclonal antibody, anti-vimentin rabbit monoclonal antibody, anti-N-cadherin rabbit monoclonal antibody (Cell Signaling, Danvers, MA), anti-α-SMA rabbit polyclonal antibody, anti-collagen I rabbit polyclonal antibody (Abcam), or anti-fibronectin mouse monoclonal antibody (BD Biosciences). The HRP‑conjugated goat anti-rabbit or anti-mouse IgG (GE Healthcare Bio-sciences) served as the secondary antibodies. The immunolabeled proteins were visualized by enhanced chemiluminescence. The band intensity was analyzed densitometrically by using ImageJ software (NIH).

Omori K., Hattori N., Senoo T., Takayama Y., Masuda T., Nakashima T., Iwamoto H., Fujitaka K., Hamada H, & Kohno N. (2016). Inhibition of Plasminogen Activator Inhibitor-1 Attenuates Transforming Growth Factor-β-Dependent Epithelial Mesenchymal Transition and Differentiation of Fibroblasts to Myofibroblasts. PLoS ONE, 11(2), e0148969.

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Hsp90α Protein Purification and Antibody Characterization

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Recombinant Hsp90α protein (rHsp90α) was purchased from Abnova (P3387; Taipei, Taiwan). Anti-Hsp90α neutralizing antibody (Hsp90α Ab, ADI-SPS-771-F) was purchased from Enzo Life Sciences, New York, NY, USA.
Antibodies used for Western blotting were anti-Hsp90α rabbit monoclonal antibody, anti-MMP-2 rabbit monoclonal antibody, anti-E-Cadherin rabbit monoclonal antibody, anti-N-Cadherin rabbit monoclonal antibody, and anti-β-actin rabbit monoclonal antibody (Cell Signaling Technology, Beverly, MA, USA).

Wang X., An D., Wang X., Liu X, & Li B. (2019). Extracellular Hsp90α clinically correlates with tumor malignancy and promotes migration and invasion in esophageal squamous cell carcinoma. OncoTargets and therapy, 12, 1119-1128.

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Antibody Profiling for Cell Characterization

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Anti‐human apoA‐I rabbit polyclonal antibody was obtained from ProteinTech. Anti‐human mast cell tryptase mouse monoclonal antibody was obtained from Chemicon International Inc. Anti‐human mast cell chymase (CC1) mouse monoclonal antibody and anti‐human β‐actin antibody were obtained from Abcam. Anti‐E‐cadherin rabbit monoclonal antibody, anti‐N‐cadherin rabbit monoclonal antibody, and anti‐vimentin rabbit monoclonal antibody were purchased from Cell Signaling Technology. Secondary biotin‐conjugated goat anti‐rabbit IgG antibody and goat anti‐mouse IgG antibody were obtained from Vector Laboratories.

Inoue Y., Okamoto T., Honda T., Nukui Y., Akashi T., Takemura T., Tozuka M, & Miyazaki Y. (2020). Disruption in the balance between apolipoprotein A‐I and mast cell chymase in chronic hypersensitivity pneumonitis. Immunity, Inflammation and Disease, 8(4), 659-671.

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