Mtor mab 7c10

Manufactured by Cell Signaling Technology

MTOR mAb (7C10) is a monoclonal antibody that recognizes the mammalian target of rapamycin (mTOR) protein. mTOR is a serine/threonine protein kinase that plays a central role in the regulation of cell growth, proliferation, and survival. This antibody can be used for the detection and analysis of mTOR in various applications, such as western blotting, immunoprecipitation, and immunohistochemistry.

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Lab products found in correlation

Gfp d5.1 mab, by Cell Signaling Technology (2 mentions) Image studio v3, by LI COR (1 mentions) Odyssey fc, by LI COR (1 mentions) Hrp conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Cd86 pe, by Thermo Fisher Scientific (1 mentions) Cd11c apc, by BD (1 mentions) Cd19 pe cy7, by BD (1 mentions) Cd80 pe, by Thermo Fisher Scientific (1 mentions) Cd11b pe cy7, by BD (1 mentions) Apc conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) B220 fitc, by BD (1 mentions) Cxcr3 apc, by Thermo Fisher Scientific (1 mentions) Pd l2 pe, by Thermo Fisher Scientific (1 mentions) Cd4 rm4 5 pe cy7, by BD (1 mentions) Pd l1 pe, by BD (1 mentions)

Close spelling variants of this product

MTOR (7C10) (12 citations) MTOR (7C10) Rabbit mAb (2 citations)

2 protocols using mtor mab 7c10

Protein Quantification in T Cells

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Equal amounts of cell lysate proteins from sorted T cells were resolved on 12% SDS-PAGE, and transferred onto PVDF membranes using standard procedures. Primary antibodies were as follows: mTOR mAb (7C10), phospho-p70 S6 kinase (Thr389) (108D2) mAb, phospho-AKT (Ser473) (D9E) mAb, and GFP (D5.1) mAb (Cell Signaling Technology). The secondary antibody was HRP-conjugated Donkey-anti-Rabbit IgG (Jackson ImmunoResearch). Data were imaged by Odyssey Fc (Li-Cor) and protein bands were analyzed by densitometry using Image Studio V3.1 (Li-Cor).

Chen W., Wan X., Ukah T.K., Miller M.M., Barik S., Cattin-Roy A.N, & Zaghouani H. (2016). Antigen-Specific Immune Modulation Targets mTORC1 Function to Drive Chemokine Receptor-Mediated T Cell Tolerance. Journal of immunology (Baltimore, Md. : 1950), 197(9), 3554-3565.

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Comprehensive Immune Cell Profiling

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The following antibodies were used: mTOR mAb (7C10), phospho-S6 (Ser235/236) (D57.2.2E)-APC, phospho-p70 S6 kinase (Thr389) (108D2) mAb, phosphor-AKT (Thr308) (C31E5E) mAb, phospho-Akt (Ser473) (D9E) mAb, GFP (D5.1) mAb, SHP-2 (D50F2) mAb (Cell Signaling Technology); APC-conjugated Donkey-anti-Rabbit IgG (Jackson ImmunoResearch); CD3e (145-2C11)-FITC, CD4 (RM4-5)-PE-Cy7, Vβ4 (KT4)-PE, CD11c-APC, CD11b-PE-Cy7, B220-FITC, CD19-PE-Cy7 and PD-L1-PE (BD Biosciences); F4/80-PE and PD-1-PE-Cy7 (BioLegend); CD71-APC and CD98-PE, CD90.1 (Thy-1.1)-PE, CXCR3-APC, CD80-PE, CD86-PE and PD-L2-PE (eBioscience); PD-1 (J43) Ab (Novus); T-bet-FITC and SH-PTP2 (C-18) (Santa Cruz Biotechnology).

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