Dried seeds of each line were de-hulled, polished and then milled into flour, according to the surging and grind-milling method described in our previous report (Xu et al., 2015 (link)), to prevent possible mineral contamination, especially by Fe. About 0.3 g of rice flour was digested with 6 ml of HNO3 and 0.2 ml of H2O2 using a microwave digestion system (Microwave300, Anton PAAR, Graz, Austria), with the following parameters: 5 min at 700 W, 700–1,200 W for 10 min, and 1,200 W for 20 min. The samples were then transferred to a block heater at 160°C for further digestion. The remaining 1 ml of digested sample was diluted with 50 ml of Milli-Q water before analysis. The Fe, Zn, Cd, Mn, Cu, and Se concentrations in the digested samples were determined using the methods described in our previous report (Xu et al., 2015 (link)). Two standards and two controls were set in each testing batch. Three replications of the tests were performed for each sample.
Microwave 300
Manufactured by Anton Paar
Sourced in Austria
The Microwave 300 is a laboratory microwave system designed for efficient sample preparation. It features a microwave cavity with a capacity of 300 mL and can operate at power levels up to 1,500 watts. The device is suitable for a range of applications that require controlled microwave heating.
Automatically generated - may contain errors
3 protocols using microwave 300
1
Mineral Content Analysis of Rice Flour
2
Scalable Synthesis and Purification of A2E
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A2E was synthesized in the dark using a commercial microwave synthesizer “Microwave 300” (Anton Paar GmbH, Austria). To scale up A2E synthesis45 all-trans retinal (100 mg equivalent to 352 µmol) and ethanolamine (9.5 µL; 155 µmol) were added in ethanol (3 mL) in the presence of acetic acid (9.3 µL; 155 µmol) using stochiometric ratios as described.29 (link) Further, large scale purification was achieved using liquid partitioning46 (link) to obtain HPLC pure A2E.
Chromatography separation and peak monitoring were achieved using an ultrahigh performance liquid chromatography (UPLC; Thermo Accela; Thermo Electron Corp., Waltham, MA, USA) system with a quaternary pump connected to an online degasser and photodiode array detector (PDA). The UPLC system was coupled with a tandem mass spectrometer (4000 Q-Trap; AB Sciex Biosystems, Foster City, CA, USA). ChromQuest software version 4.1 (San Jose, CA, USA) was used to control all parameters of UPLC. For analytical separations, an Xterra RP 18 column was used (4.6 × 150 mm with 5 µm particle size; Waters, Milford, MA, USA). Ionization of analytes was accomplished by electrospray ionization (ESI; TurboIon Spray; ABSciex) operating in the positive ion mode followed by tandem mass spectrometric analysis. Data acquisition and integration were performed by Analyst 1.5.2 software (ABS Biosystems, Foster City CA, USA).
Chromatography separation and peak monitoring were achieved using an ultrahigh performance liquid chromatography (UPLC; Thermo Accela; Thermo Electron Corp., Waltham, MA, USA) system with a quaternary pump connected to an online degasser and photodiode array detector (PDA). The UPLC system was coupled with a tandem mass spectrometer (4000 Q-Trap; AB Sciex Biosystems, Foster City, CA, USA). ChromQuest software version 4.1 (San Jose, CA, USA) was used to control all parameters of UPLC. For analytical separations, an Xterra RP 18 column was used (4.6 × 150 mm with 5 µm particle size; Waters, Milford, MA, USA). Ionization of analytes was accomplished by electrospray ionization (ESI; TurboIon Spray; ABSciex) operating in the positive ion mode followed by tandem mass spectrometric analysis. Data acquisition and integration were performed by Analyst 1.5.2 software (ABS Biosystems, Foster City CA, USA).
3
Synthesis and Characterization of Fluorinated Aryl Diarylbenzenes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents and dry solvents were purchased from commercial sources and used as received. 1 H, 13 C{ 1 H} and 19 F{ 1 H} NMR spectra were recorded on a Bruker DPX-400 spectrometer (400 MHz, 100 MHz, and 367.5 MHz, respectively) and a JEOL JMN-ECZ400S spectrometer (400 MHz and 100 MHz, respectively) in a deuterated solvent using TMS as an internal standard. For 19 F{ 1 H} NMR, the chemical shift of hexafluorobenzene (δ = -163.0 ppm in CDCl 3 ) was used as an external reference. The assignments of the 13 C{ 1 H} NMR were performed by DEPT experiments. In cases of 10Ar-DBAs 10, NMR spectra were measured using a mixture of diastereomer A and B (A/B = 51/49-56/44). Hence, integral values of 1 H NMR were shown using H A and H B , which were belonged to isomer A and B, respectively. Assignments of 13 C NMR were also shown using C A and C B , which were belonged to isomer A and B, respectively. IR spectra were recorded on a JASCO FT/IR-4200 spectrometer equipped with an ATM detector. High-resolution mass spectra were obtained on an AB SCEIX Triplet TOF 4600 mass spectrometer. Melting points were recorded on an SRS-Optimelt automated melting point system and were uncorrected. Microwave heating was performed by Anton-Paar Microwave 300 (850 W, 2455 MHz) using 10 mL glass vessel.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!