Dspe peg

Manufactured by Merck Group

Sourced in United States

DSPE-PEG is a lipid-based compound that consists of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) covalently linked to polyethylene glycol (PEG). It is commonly used as a component in the formulation of liposomes and other lipid-based drug delivery systems.

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Close spelling variants of this product

DSPE-PEG-MAL Distearoyl-sn-glycero-3-phosphoethanolamine-PEG (DSPE-PEG), (DSPE-PEG-COOH),

5 protocols using dspe peg

Siglec-7 Targeted Liposomal Delivery

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The liposomes were prepared as described (25 (link)). Briefly, 4 mol% Siglec-7 targeted liposomes were composed of distearoyl phosphatidylcholine (DSPC, Avanti Polar Lipids): cholesterol (Chol, Sigma-Aldrich): polyethyleneglycol-distearoyl phosphoethanolamine (PEG-DSPE, NOF): Siglec-7 ligand-lipid (35 (link)) in a 58: 37: 1: 4 molar ratio. Naked liposomes were composed of 5mol% PEG-DSPE instead of the Siglec-7 ligand-lipid. Antigenic liposomes substituted 1 mol% DSPC for 1 mol% C80 GMM. Fluorescent liposomes contained 0.2 mol% of Alexa fluor 647-PEG-DSPE (28 (link)). For liposome preparation, lipid components in dimethyl sulfoxide (Siglec-7 ligand-lipid, C80 GMM, and Alexa 647-PEG-DSPE) were mixed and lyophilized in a glass tube. The other components in chloroform were then added to the tube and dried completely by air flow. The dried lipids were hydrated with 1 mL of PBS, sonicated, then extruded by an liposome extruder (Avanti Polar Lipids) until the size became around 100 nm measured by Zetasizer (Malvern). The GMM concentration in the antigenic liposomes was 10 μM.

Kawasaki N., Rillahan C.D., Cheng T.Y., Van Rhijn I., Macauley M.S., Moody D.B, & Paulson J.C. (2014). Targeted delivery of mycobacterial antigens to human dendritic cells via Siglec-7 induces robust T cell activation. Journal of immunology (Baltimore, Md. : 1950), 193(4), 1560-1566.

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Gd-Loaded Liposomes Synthesis and Characterization

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Gadolinium diethylenetriaminepentaacetate (Gd; Sigma-Aldrich) was incorporated into 100-nm liposomes. A lipid mixture of hydrogenated soybean phosphatidylcholine (HSPC; Avanti) cholesterol (Sigma-Aldrich), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl (Rhod-DSPE; Avanti) and PEG-DSPE (Avanti) at a molar ratio of 55.94:39:0.06:5 respectively were dissolved in absolute ethanol at 70°C. The dissolved lipids were quickly injected into a PBS solution containing 270 mg/ml of Gd, at 70°C, to reach a lipid concentration of 50mM. The liposomes were extruded five times through 100-nm polycarbonate membranes (Whatman) at 70°C. The free Gd was removed by dialysis in a 12-14kDa membrane (Spectrum Laboratories) against PBS solution at 4°C for 24 hours. Liposomes were sized using a Zetasizer NanoZSP (Malvern).

Zinger A., Koren L., Adir O., Poley M., Alyan M., Yaari Z., Noor N., Krinsky N., Simon A., Gibori H., Krayem M., Mumblat Y., Kasten S., Ofir S., Fridman E., Milman N., Lübtow M.M., Liba L., Shklover J., Shainsky-Roitman J., Binenbaum Y., Hershkovitz D., Gil Z., Dvir T., Luxenhofer R., Satchi-Fainaro R, & Schroeder A. (2019). Collagenase Nanoparticles Enhance the Penetration of Drugs into Pancreatic Tumors. ACS nano, 13(10), 11008-11021.

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Lipid-based Nanoparticle Formulation

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L-α-phosphatidylcholine, cholesterol, DSPE-PEG (2000), EGCG and quercetin were obtained from Sigma-Aldrich, St. Louis, MO, USA. Chloroform and methanol were obtained from Merck. Milli Q water and phosphate buffer from sigma was used where ever necessary.

Das A., Konyak P.M., Das A., Dey S.K, & Saha C. (2019). Physicochemical characterization of dual action liposomal formulations: anticancer and antimicrobial. Heliyon, 5(8), e02372.

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Lipid-based Nanoparticle Formulation

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The 1,2-distearoryl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol-2000)] ammonium salt (DSPE-PEG), dioleoyl phosphatidic acid (DOPA) and 1,2-dioleoyl-3-trimethylammonium-propane chloride salt (DOTAP) were purchased from Avanti Polar Lipids (Alabaster, AL). The DSPE-PEG-AEAA was synthesized according to previous publication of our lab.^{58 (link)} Doxorubicin, cholesterol and protamine were purchased from Sigma-Aldrich (St. Louis, MO). DiI was purchased from ThermoFisher Scientific (Waltham, MA).

Liu Q., Zhu H., Tiruthani K., Shen L., Chen F., Gao K., Zhang X., Hou L., Wang D., Liu R, & Huang L. (2018). Nanoparticle-Mediated Trapping of Wnt Family Member 5A in Tumor Microenvironments Enhances Immunotherapy for B-Raf Proto-Oncogene-Mutant Melanoma. ACS nano, 12(2), 1250-1261.

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Lipid Mixture Characterization Protocol

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DPPC, MPPC, 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1-palmitoyl-2-{6-[(7-nitro-2-1,3-benzoxadiazol-4-yl) amino]hexanoyl}-sn-glycero-3-phosphocholine (NBD-PC), cholesterol (chol), and 3β-[N-(N′,N′-dimethylaminoethane)-carbamoyl]cholesterol (DC-chol) were obtained from Avanti Polar Lipids (Alabaster, AL). DSPE-PEG (average PEG molecular mass of 2,000 amu), fluorescamine (4-phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione), HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) sodium salt, sodium chloride (NaCl), citrate-phosphate-dextrose solution (CPD), Triton X-100 (TX100), sulforhodamine B sodium salt (SRB, ~75% purity), and trichloro acetic acid (TCA) were acquired from Sigma Aldrich (St. Louis, MO). TA (4-(aminomethyl)cyclohexane-1-carboxylic acid) and 5(6)-carboxyfluorescein (CF) were purchased from Fluka (Buchs, Switzerland). Perchloric acid, ammonium heptamolybdate tetrahydrate, acetic acid, and tris(hydroxymethyl) aminomethane (TRIS) were purchased from Merck KgaA (Darmstadt, Germany). Ascorbic acid was obtained from Acros Organics (Geel, Belgium). TCA was dissolved in MilliQ at a 50% (w/v) concentration and stored at 4 °C. SRB was dissolved in 1% acetic acid at a concentration of 0.4% (w/v). TRIS was dissolved in MilliQ at a 10-mM concentration without further pH adjustments. All chemicals used were analytical grade.

van Raath M.I., Weijer R., Nguyen G.H., Choi B., de Kroon A.I, & Heger M. (2016). Tranexamic Acid-Encapsulating Thermosensitive Liposomes for Site-Specific Pharmaco-Laser Therapy of Port Wine Stains. Journal of biomedical nanotechnology, 12(8), 1617-1640.

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