H9, HH, C5MJ, J. Cam 1.6, SUP-T1, Tib152, and CCL119 are TCL cell lines purchased from the ATCC. SU-DHL6, SU-DHL2, Jeko-1, JVM-2, Z-138, Rec-1 are characterized BCL lines purchased from the ATCC. DND41 is a T-cell line purchased from Deutsche Sammlung von Mikroorganism und Zellkulturen GmbH (DSMZ; Braunschweig, Germany). OCI-LY10 and OCI-LY7 are BCL cell lines from DSMZ. All cell lines were grown in RPMI-1640 (H9, HH, J. Cam 1.6, SUP-T1, Tib-152, CCL119, DND41, Jeko-1, JVM-2, Z-138, Rec-1, SU-DHL6, and SU-DHL2) or IMDM media (C5MJ, OCI-LY10, and OCI-LY7) with 10% FBS (Life Technology) and maintained at a concentration of 0.3 × 106 cells/mL. All cell lines were authenticated from a hematopathologist, including verification of morphology and immunophenotype (11 (link)–13 (link)).
Su dhl2
Manufactured by Thermo Fisher Scientific
The SU-DHL2 is a laboratory equipment designed for sample preparation and handling. It provides basic functions for sample processing, such as mixing, agitation, and incubation. The device features a compact and durable construction to support standard laboratory workflows.
Automatically generated - may contain errors
Lab products found in correlation
Su dhl 2, by American Type Culture Collection (2 mentions)
Oci ly7, by Leibniz Institute DSMZ (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Z 138, by American Type Culture Collection (1 mentions)
Sup t1, by American Type Culture Collection (1 mentions)
Jvm 2, by American Type Culture Collection (1 mentions)
Tib 152, by American Type Culture Collection (1 mentions)
Supt1, by Thermo Fisher Scientific (1 mentions)
Su dhl 6, by American Type Culture Collection (1 mentions)
Jeko 1, by American Type Culture Collection (1 mentions)
Ccl 119, by American Type Culture Collection (1 mentions)
Rec 1, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Oci ly10, by Leibniz Institute DSMZ (1 mentions)
Oci ly10, by American Type Culture Collection (1 mentions)
Iscove s modified dulbecco s medium, by Thermo Fisher Scientific (1 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Nci h929, by Thermo Fisher Scientific (1 mentions)
β mercaptoethanol, by Thermo Fisher Scientific (1 mentions)
Rpmi 8226, by Leibniz Institute DSMZ (1 mentions)
2 protocols using su dhl2
1
TCL and BCL Cell Line Characterization
2
Culturing and Sourcing Multiple Myeloma Cell Lines
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Cell lines L363, OPM-2, MM1.S, RPMI-8226, and SU-DHL-2 were cultured in RPMI 1640 GlutaMAX HEPES culture medium (Life Technologies) supplemented with 10% fetal bovine serum (FBS, Biowest) and 100 µg/ml penicillin–streptomycin (Gibco/Life Technologies). UM9 was cultured with 20% FBS, and NCI-H929 with 20% FBS, 1 mM sodium pyruvate (Thermo Fisher), and 50 µM β-mercaptoethanol (Life Technologies). OCI-Ly1, OCI-Ly3, OCI-Ly7, and OCI-Ly10 were cultured in Iscove’s modified Dulbecco’s medium (Life Technologies) supplemented with 20% FBS and 100 µg/ml penicillin–streptomycin. Cell lines L363, RPMI-8226, OPM-2, OCI-Ly3, OCI-Ly7, OCI-Ly1 and NCI-H929 were purchased from DSMZ, MM1.S, OCI-Ly10, and SU-DHL-2 were purchased from ATCC. Cell line UM9 was derived from an MM patient at our UMCU hospital). All cell lines were tested negative for mycoplasma before and during the experiments. Primary MM cell samples were derived from patients diagnosed at the Academic Medical Center, Amsterdam, The Netherlands. This study was conducted and approved by the AMC Medical Committee on Human Experimentation. Informed consent was obtained in accordance with the Declaration of Helsinki.
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