The surface morphology of the microcapsules was analyzed by means of a Zeiss Ultra 55 field emission scanning electron microscope (FE-SEM) (Oberkochen, Germany). The powders were placed onto conductive tape on metal studs and sputter-coated with a platinum layer during 10 s using a Leica EM MED020 sputter coater. The FE-SEM images were taken at 22 °C with a 1 kV voltage. The particle dimensions were measured from the scanning electron microscope images (5000×) at random locations (n = 200) using the ImageJ® software v1.52a (National Institutes of Health, USA).
Em med020 sputter coater
Manufactured by Leica
Sourced in Germany, Spain
The EM MED020 is a sputter coater manufactured by Leica. It is designed to deposit thin conductive coatings on specimens for scanning electron microscopy (SEM) and other applications that require conductive surfaces. The device uses a controlled flow of inert gas to sputter-coat the specimen with a thin layer of metal, typically gold or carbon, to enhance surface conductivity and improve image quality in SEM imaging.
Automatically generated - may contain errors
Lab products found in correlation
Ultra 55, by Zeiss (1 mentions)
Geminisem 500, by Zeiss (1 mentions)
Jem 2100f, by JEOL (1 mentions)
Moticam 580 5.0mp, by Motic (1 mentions)
Ba410e, by Motic (1 mentions)
Images plus 3, by Motic (1 mentions)
Geminisem 500 microscope, by Zeiss (1 mentions)
Jsm 5410, by JEOL (1 mentions)
Bx50 light microscope, by Olympus (1 mentions)
Fesem ultra55, by Zeiss (1 mentions)
10 protocols using em med020 sputter coater
1
Microcapsule Surface Morphology Characterization
2
SEM and TEM Analysis of PPy Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The surface and cross-section morphology of the samples were analysed in a GeminiSEM 500 high-resolution field-emission scanning electron microscope (HR-FESEM) (Carl Zeiss Microscopy, Jena, Germany) with an accelerating voltage of 0.8–1.0 kV. The samples were coated with a platinum layer by an EM MED020 sputter coater (Leica, Wetzlar, Germany). The cross-section was observed in samples previously immersed in liquid N2 and cryofractured. The morphology of PPy nanoparticles was observed by HR-FESEM and a TEM (transmission electron microscope JEM 2100F operated at 200 kV, JEOL, Tokyo, Japan). Previously, the nanoparticles were dispersed in an ultrasound bath for 1h. One drop was then placed on a SEM sample holder and carbon-coated TEM grid for 2 h to ensure complete drying. An estimation of the size distribution of PPy nanoparticles was obtained from HR-FESEM micrographs with ImageJ software. The diameter of about 100 PPy nanoparticles (only those that show clear spherical shape) was measured to obtain the average diameter and diameters’ distribution.
3
Morphological Analysis of Non-Woven Fabrics Treated with Cranberry Extracts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The morphology of the non-woven fabrics treated with and without cranberry extracts was observed by optical microscopy (Motic BA410E) and was photographed at 10× and 40× magnifications with the Moticam 580 5.0MP. The images were processed by the Motic Images Plus 3.0 software (Motic, Barcelona, Spain). Macroscopic photographs of the fabrics were also performed with a 64MP Xiaomi camera with a Sony IMX682 sensor and a f/1.89 opening. Scanning electron microscopy was used to analyze in greater detail the morphology of the fibers of the non-woven fabric (porous structure). Untreated and treated non-woven fabrics samples were observed at a magnification of ×100 and ×1000 with a GeminiSEM 500 high-resolution field-emission scanning electron microscope (HR-FESEM) (Zeiss-Oxford Instruments, Abingdon, UK) with an accelerating voltage of 1.5 kV. The samples were previously coated with a platinum layer by an EM MED020 sputter coater (Leica).
4
FE-SEM Analysis of Surface Topology
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The surface topology was analyzed by field-emission scanning electron microscopy (FE-SEM) in a Zeiss Ultra 55 equipment (Oberkochen, Germany). They were prepared in two ways: either small pieces of the films (1 cm diameter) were placed on the sample holders with double-sided adhesive carbon tape; or aqueous low-concentrated dispersions (2 mg·L−1) of the different samples were prepared, sonicated, and directly deposited onto clean circular coverslips, and water evaporation was allowed overnight. Then, all the samples were sputter-coated with a platinum layer for 15 s using a Leica EM MED020 sputter coater (Wetzlar, Germany). Finally, the micrographs were taken using a working distance between 3 and 4 mm, a voltage of 2 kV, and magnification of 100,000×. The size and distribution of the different samples were measured from the average of random locations (n = 100) through the software Image J® 1.52p.
5
Degradation Study of PHBV with GO and CNFs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The qualitative results of the degradation study were evaluated analyzing the morphology (surface and cross-section) of neat PHBV and PHBV with 1% w/w of GO and CNFs by high-resolution field emission scanning electron microscope (HRFESEM) using a GeminiSEM 500 microscope (Carl Zeiss, Jena, Germany) at the beginning and at the end of the degradation time (0 and 3 months). The samples were previously coated with a platinum layer by an EM MED020 sputter coater (Leica, Wetzlar, Germany). The cross-section was observed after cryofracture.
6
FESEM Fractured Surface Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cross-sectional areas of the fractured samples in the impact test were observed by using field-emission scanning electron microscopy (FESEM), model ZEISS ULTRA 55 from Oxford Instruments (Tubney Woods, Abingdon, Oxfordshire, UK). The samples were first coated with a gold layer under vacuum conditions to increase their electrical conductivity by employing an EM MED020 sputter coater from Leica Microsystems (Wetzlar, Germany). All fractured surfaces of the samples were observed using an accelerating voltage of 2 kV.
7
SEM Analysis of Fractured Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the impact test, the fractured samples were taken to observe their surfaces in a field emission scanning electron microscope (FESEM) model Zeiss ULTRA from Oxford Instruments (Abingdon, UK) with a voltage of 2 kV. Before observation, the samples were coated with a thin layer of gold and platinum using an EM MED020 sputter coater from Leica Microsystems (Wetzlar, Germany).
8
SEM Analysis of Bio-HDPE Fracture Surfaces
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The surface morphology of fractured samples in the Charpy Impact test of Bio-HDPE with CSF treated and untreated were analyzed with a Zeiss Ultra 55 Field Emission Scanning Electron Microscope (FESEM) supplied by Oxford Instruments (Oxfordshire, UK). All fractured surfaces were coated for 120 s with a thin layer of Au-Pd alloy. This process was carried out under vacuum conditions using an EM MED020 sputter coater from Leica Microsystems (Wetzlar, Germany) following the methodology employed by Quiles-Carillo et al. [54 (link)]. All samples were observed with an accelerating voltage of 2 kV.
9
Cryo-fractured Monolayer and Bilayer Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cross-sections of the monolayers and bilayers were observed by field-emission scanning electron microscopy (FESEM) in a JEOL model JSM-5410 (Tokyo, Japan). The samples were cryo-fractured in liquid nitrogen, mounted on the observation holders using double-sided carbon tape, and covered with a platinum layer (EM MED020 sputter coater, Leica Biosystems, Barcelona, Spain). An acceleration voltage of 2.0 kV was used, and the layer thicknesses were determined using the ImageJ v1.53c Program.
10
Microstructural Analysis of Polymer Films
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The determination of the number of layers and each layer thickness was performed by image analysis using light optical microscopy (Olympus BX50 light microscope, Tokyo, Japan) using 50× magnification. The samples were cross-sectioned with a microtone (Microtone M240, Especialidades Médicas Myr, S.L., Tarragona, Spain) and stained with an iodopovine solution (10 g/100 mL, Viatris Inc., Madrid, Spain).
The microstructure and surface of the films were observed by Field Emission Scanning Electron Microscope (FESEM, Ultra 55, Zeiss, Oxford Instruments, Abingdon, UK). To this end, the samples were mounted on holders using double-sided carbon tape, coated with a platinum layer (EM MED020 sputter coater, Leica Biosystems, Barcelona, Spain), and observed using an accelerated voltage of 2 kV. The film samples were previously cryo-fractured with liquid nitrogen to obtain their cross-sections.
The microstructure and surface of the films were observed by Field Emission Scanning Electron Microscope (FESEM, Ultra 55, Zeiss, Oxford Instruments, Abingdon, UK). To this end, the samples were mounted on holders using double-sided carbon tape, coated with a platinum layer (EM MED020 sputter coater, Leica Biosystems, Barcelona, Spain), and observed using an accelerated voltage of 2 kV. The film samples were previously cryo-fractured with liquid nitrogen to obtain their cross-sections.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!