Acd40

Manufactured by BioLegend

ACD40 is a lab equipment product offered by BioLegend. It is a device used for the detection and analysis of the CD40 antigen, a protein involved in the immune system's cellular signaling processes.

Automatically generated - may contain errors

Lab products found in correlation

Tlrl 1826, by InvivoGen (1 mentions) Negative selection mouse b cell isolation kit, by STEMCELL (1 mentions) Cpg odn 1826, by InvivoGen (1 mentions) Tlrl r848, by Merck Group (1 mentions) Anti igg3, by BD (1 mentions) Recombinant murine il 5, by Thermo Fisher Scientific (1 mentions) Mouse recombinant il 4, by Merck Group (1 mentions) Lipopolysaccharides (lps), by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Tgf β, by Thermo Fisher Scientific (1 mentions) Tgf β, by R&D Systems (1 mentions) Anti igg2b, by BioLegend (1 mentions)

2 protocols using acd40

B Cell Activation via Immunostimulants

Check if the same lab product or an alternative is used in the 5 most similar protocols

B cells were isolated from Il12a^GFP and Ebi3^Tom mouse spleens using a negative selection mouse B cell isolation kit (StemCell Technologies, #19854). B cells were seeded in a 48-well plate (1e⁶/ml) in RPMI containing 10% heat-inactivated serum, 1x pen/strep, and B-mercaptoethanol. Cells were stimulated for 48 hours using the combinations of the following reagents: aIgM (10ug/ml; Jackson Immune Research #115–006-075), aCD40 (1ug/ml; Biolegend #102901), R848 (1ug/ml; Invivogen # tlrl-r848), LPS (1ug/ml; Sigma # 437627), CpG ODN1826 (1ug/ml; Invivogen # tlrl-1826).

Michaud D., Mirlekar B., Bischoff S., Cowley D.O., Vignali D.A, & Pylayeva-Gupta Y. (2019). Pancreatic cancer-associated inflammation drives dynamic regulation of p35 and Ebi3.. Cytokine, 125, 154817.

+ Open protocol

+ Expand

Induction of Class-Switch Recombination

Check if the same lab product or an alternative is used in the 5 most similar protocols

CH12 cells were stimulated to undergo CSR to IgA by treatment with 1-5 µg/mL aCD40 (BioLegend), 5 ng/ml TGFb (R&D Systems) and 5 ng/ml of mouse recombinant IL-4 for 48 h. B lymphocytes were stimulated to undergo class switching with 5 µg/ml LPS (Sigma-Aldrich) and 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich) for CSR to IgG1; 5 µg/ml LPS only for CSR to IgG3; 5 µg/ml LPS, 10 ng/ml BAFF (PeproTech) and 2 ng/ml TGFb for CSR to IgG2b; or 5 µg/ml LPS, 10 ng/ml BAFF, 2 ng/ml TGFb and 1.5 ng/ml recombinant murine IL-5 (PeproTech) for CSR to IgA. For class switching analysis, cell suspensions were stained with fluorochrome-conjugated anti-IgG1, anti-IgG3 (BD-Biosciences), anti-IgG2b (BioLegend), or anti-IgA (Southern Biotech).

Delgado-Benito V., Berruezo-Llacuna M., Altwasser R., Winkler W., Balasubramanian S., Sundaravinayagam D., Graf R., Rahjouei A., Driesner M., Jung L.A., Janz M., Akalin A., & Virgilio M.D. (2020). Pdap1 protects mature B lymphocytes from stress-induced cell death and promotes antibody gene diversification.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!