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Human oncostatin m

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Human Oncostatin M is a recombinant protein that serves as a cytokine and growth factor. It is involved in the regulation of cell growth and differentiation.

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Lab products found in correlation

Dmem high glucose media, by Thermo Fisher Scientific (1 mentions) Mtesr1, by Corning (1 mentions) Mtesrtm1, by STEMCELL (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Matrigel, by Corning (1 mentions) Hepatozyme sfm, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions) Mouse recombinant wnt3a, by Thermo Fisher Scientific (1 mentions)

2 protocols using human oncostatin m

1

Hepatocyte and Biliary Epithelial Cell Induction

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For induction of hepatocytes, liver progenitor cells were collected and seeded onto collagen type I-coated plates at 2 × 104 cells/cm2. The cells were cultured for 2 days in SHM medium and the medium was supplemented with 1 μM Dexamethasone (Dex) (Sigma) and 20 ng/ml human Oncostatin M (PeproTech) for 6 days. For induction of biliary epithelial cells, the liver progenitor cells were isolated and co-cultured with pre-inoculated low density mouse embryonic fibroblasts (passage 2). The mouse embryonic fibroblasts were isolated from E13.5 C57BL/6 J mouse embryos and cultured in DMEM high-glucose media (Gibco) supplemented with 10% FBS (Ausbian). The medium was replaced with mTeSRTM1 (STEMCELL Technologies) containing YAC (mTeSR1+YAC), and on day 6, the medium was replaced with mTeSR1+YAC supplemented with 2% Matrigel (Corning) for another 6 days. All media were changed every other day.
Yuan Y., Wang C., Zhuang X., Lin S., Luo M., Deng W., Zhou J., Liu L., Mao L., Peng W., Chen J., Wang Q., Shu Y., Xue Y, & Huang P. (2022). PIM1 promotes hepatic conversion by suppressing reprogramming-induced ferroptosis and cell cycle arrest. Nature Communications, 13, 5237.
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2

Hepatic Differentiation Protocol

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RPMI, 50× B27 Supplement, Knockout DMEM (KO-DMEM), Knockout Serum Replacement Medium (KO-SR), GlutaMAX, Penicillin/Streptomycin (P/S), and HepatoZYME-SFM (HZM) were purchased from Life Technologies. Recombinant Mouse Wnt3a, Human Activin A (AA), Human Hepatocyte Growth Factor (HGF), and Human Oncostatin M (OSM) were from PeproTech (Hannoun et al., 2010; Hay et al., 2011; Szkolnicka et al., 2013 ).
Zhou X., Sun P., Lucendo-Villarin B., Angus A.G., Szkolnicka D., Cameron K., Farnworth S.L., Patel A.H, & Hay D.C. (2014). Modulating Innate Immunity Improves Hepatitis C Virus Infection and Replication in Stem Cell-Derived Hepatocytes. Stem Cell Reports, 3(1), 204-214.
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