Splenocytes and tumor-infiltrating lymphocytes (TILs) isolated from treated mice were cocultured with 10,000 K7M2 cells. K7M2 cells were left uninfected or infected with Delta-24-RGD or Delta-24-ACT at an MOI of 100 MOI. Four hours later, K7M2 cells were stimulated with recombinant mouse IFN-gamma protein and were collected and fixed with 4% formaldehyde 48 h later. After 72 h of coculture, supernatants were collected, and IFNgamma ELISA (R&D System, Inc., Minneapolis, MN) and MACS Plex Mouse Cytokine 10 kits (Miltenyi Biotec, Bergisch Gladbach, Germany) were used for analysis following the manufacturers’ instructions.
Macs plex mouse cytokine 10 kits
Manufactured by Miltenyi Biotec
Sourced in Germany
The MACS Plex Mouse Cytokine 10 kits provide a multiplex analysis of 10 different mouse cytokines in a single sample. The kits utilize magnetic bead-based technology to enable simultaneous quantification of multiple cytokines using flow cytometry.
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Lab products found in correlation
2 protocols using macs plex mouse cytokine 10 kits
1
Cytokine Profiling of Oncolytic Virus-Treated Tumors
2
Assessing Cytokine Responses in Splenocytes and TILs
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Splenocytes and tumor-infiltrating lymphocytes (TILs) isolated from treated mice were cocultured with 10,000 K7M2 cells. K7M2 cells were left uninfected or infected with Delta-24-RGD or Delta-24-ACT at an MOI of 100 MOI. Four hours later, K7M2 cells were stimulated with recombinant mouse IFNγ protein and were collected and fixed with 4% formaldehyde 48 hours later. After 72 hours of coculture, supernatants were collected, and IFNγ ELISA (R&D Systems, Inc.) and MACS Plex Mouse Cytokine 10 kits (Miltenyi Biotec, Bergisch Gladbach) were used for analysis following the manufacturers’ instructions.
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