The PaD2HGDH pET20b(+) plasmid harboring the PA0317 gene was designed in-lab and purchased from GenScript. The plasmid was sequenced to verify the presence of the wildtype gene. E. coli strain Rosetta(DE3)pLysS was from Novagen. Bovine serum albumin was purchased from Promega. Luria–Bertani (LB) agar, LB broth, chloramphenicol, IPTG, lysozyme, sodium hydrosulfite (dithionite), PMS, and PMSF were obtained from Sigma–Aldrich. Ampicillin was purchased from ICN Biomedicals. d -2-hydroxyglutarate was purchased from MilliporeSigma. d -malate was purchased from Alfa Aesar. EDTA, glycerol, and all other reagents were of the highest purity commercially available.
Pad2hgdh pet20b plasmid
Manufactured by GenScript
The PaD2HGDH pET20b(+) plasmid is a recombinant DNA vector designed for the expression of the D2HGDH protein in Escherichia coli. The plasmid contains the necessary genetic elements, including the T7 promoter and terminator, to facilitate the production of the target protein.
Automatically generated - may contain errors
Lab products found in correlation
Bovine serum albumin (bsa), by Promega (2 mentions)
Ampicillin, by ICN Biomedicals (2 mentions)
D malate, by Thermo Fisher Scientific (2 mentions)
Lysozyme, by Merck Group (2 mentions)
Isopropyl β d 1 thiogalactopyranoside (iptg), by Merck Group (2 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Merck Group (2 mentions)
Chloramphenicol, by Merck Group (2 mentions)
Luria bertani agar, by Merck Group (2 mentions)
D 2 hydroxyglutarate, by Merck Group (2 mentions)
Lb broth, by Merck Group (1 mentions)
Sodium hydrosulfite, by Merck Group (1 mentions)
Luria bertani broth, by Merck Group (1 mentions)
Sodium deuteroxide, by Cambridge Isotopes (1 mentions)
Deuterium chloride, by Cambridge Isotopes (1 mentions)
Deuterium oxide, by Cambridge Isotopes (1 mentions)
2 protocols using pad2hgdh pet20b plasmid
1
Cloning and Purification of PaDGHD
2
Recombinant PA0317 Enzyme Production
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The PaD2HGDH pET20b(+) plasmid harboring the PA0317 gene was designed in-lab and purchased from GenScript. The plasmid was sequenced to verify the presence of the wildtype gene. Escherichia coli strain Rosetta(DE3)pLysS was from Novagen. Bovine serum albumin was purchased from Promega. Luria–Bertani agar, Luria–Bertani (LB) broth, chloramphenicol, IPTG, lysozyme, PMS, and PMSF were obtained from Sigma–Aldrich. Ampicillin was purchased from ICN Biomedicals. d -2-hydroxyglutarate was purchased from MilliporeSigma. D-malate was purchased from Alfa Aesar. Deuterium oxide, deuterium chloride, and sodium deuteroxide were purchased from Cambridge Isotope Laboratories, Inc. Glucose, glycerol, and all the other reagents were of the highest purity commercially available.
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