Abi 7500 fast real time cycler

Manufactured by Thermo Fisher Scientific

Sourced in United States

The ABI 7500 FAST real-time cycler is a laboratory instrument designed for fast, precise, and reliable real-time PCR analysis. It is capable of performing rapid thermal cycling and data acquisition, enabling efficient and high-throughput nucleic acid quantification.

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Lab products found in correlation

Taqman fast pcr reagent, by Thermo Fisher Scientific (2 mentions) Nuclisens easymag instrument, by bioMérieux (1 mentions) Caspase 8, by Thermo Fisher Scientific (1 mentions) Rneasy plus kit, by Qiagen (1 mentions) High capacity abi rt pcr kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

ABI 7500 (1035 citations) ABI 7500 Fast (145 citations) 7500 Fast (121 citations) ABI 7500 cycler (27 citations) 7500 real-time cycler (14 citations) 7500 Fast Cycler (12 citations) ABI 7500 real-time cycler (6 citations) ABI 7500 Fast cycler (6 citations) 7500 Fast Real-Time (6 citations) ABI 7500 Fast Real-Time PCR cycler (5 citations)

3 protocols using abi 7500 fast real time cycler

Molecular detection of enteric and respiratory viruses

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A volume of 200 μL of transport medium was extracted by using the Specific B protocol on the NUCLISENS easyMAG instrument (bioMérieux, Marcy l'Etoile, France). The elution volume was 50 μL. The amplification step was performed immediately after extraction, without freezing of nucleic acids. Ten microlitres of extract was mixed with ready-to-use commercial mastermix, and one-step reverse transcription and quantitative PCR (RT-qPCR) reactions were performed on an ABI7500fast real-time cycler (Applied Biosystems, Foster City, CA, USA), according to the manufacturers' recommendations. The enteric viruses (RV and NV) were detected by using KHRV and KHPNOV kits from Ceeram (La Chapelle-sur-Erdre, France); the respiratory viruses (influenza A and B viruses, RSV and hMPV) were detected by using the MWS kits from bioMérieux [22] (link). The molecular results were analysed in a masked manner to the results of the behavioural survey.

Pillet S., Berthelot P., Gagneux-Brunon A., Mory O., Gay C., Viallon A., Lucht F., Pozzetto B, & Botelho-Nevers E. (2015). Contamination of healthcare workers' mobile phones by epidemic viruses. Clinical Microbiology and Infection, 22(5), 456.e1-456.e6.

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Quantifying Influenza Gene Expression in Human Airway Cells

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RNA was isolated from HBEpCs using the RNeasy plus kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions, and 1.0 μg RNA was used to generate cDNA with the High capacity ABI-RT PCR kit (Applied Biosystems). RT-PCR reactions were done using the TaqMan FAST PCR reagent (Applied Biosystems) and the ABI 7500 FAST real-time cycler (Applied Biosystems). TaqMan primers for FADD, TNFRSF1, Caspase-3, Caspase-8, and glyceraldehyde phosphate dehydrogenase (GAPDH) were purchased from Thermo Fisher Scientific (Thermo Fisher Scientific, Waltham, MA). Influenza matrix gene expression was quantified with a standard curve and reported as copy numbers of IAV. Gene expression was normalized to GAPDH and reported as fold change. The change in gene expression was calculated thus: fold change = 2^(ddCt), ddCt is the dCt (H1N1-infected) − dCt (mock-infected), where dCt = Ct (detected gene) − Ct (GAPDH) and Ct is the threshold number.

Othumpangat S., Beezhold D.H, & Noti J.D. (2018). Influenza virus infection modulates the death receptor pathway during early stages of infection in human bronchial epithelial cells. Physiological genomics, 50(9), 770-779.

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Real-Time PCR Analysis of Viral Gene Expression

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Real time PCR reactions were carried out using the TaqMan FAST PCR reagent (Applied Biosystems) and the ABI 7500 FAST real-time cycler (Applied Biosystems). TaqMan primers for COX6C, Caspase-9, and glyceraldehyde phosphate dehydrogenase (GAPDH) were purchased from Applied Biosystems. Gene expression was normalized using GAPDH as the housekeeping gene, and is reported as fold change. The change in gene expression was calculated using the formula: fold change=2^−(ddCt), ddCt=dCt (H1N1 infected) - dCt (mock infected), where dCt=Ct (detected gene) - Ct (GAPDH) and Ct is the threshold number).

Othumpangat S., Noti J.D, & Beezhold D.H. (2014). Lung epithelial cells resist influenza A infection by inducing the expression of cytochrome c oxidase VIc which is modulated by miRNA 4276. Virology, 0, 256-264.

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