C57BL/6 mice (8 weeks, 18–22 g) were purchased from Jackson Laboratories and housed in the Biomedical Sciences Building at OUHSC. The OUHSC IACUC approved all experiments. Plasmodium yoelii (clone 17XNL, obtained from MR4, ATCC) was routinely passaged through mosquitoes and mouse infections were initiated by serial transfer of 106 parasite-infected red blood cells via tail vein injection. Parasitemia was measured using flow cytometry as described (Malleret et al., 2011 (link)). Giemsa staining of thin blood smears was done in parallel. At the indicated times, mice were injected i.p. with 200 μg α-CD4 (GK1.5), 500 μg of α-IFN-γ (XMG1.2), 200 μg α-PD-L1 (10F.9G2), 50 μg of α-OX40 Ab (OX86), 200 μg α-PD-L1 and 50 μg α-OX40, or 200 μg α-PD-1 (RMP1-14) and 50 μg α-OX40, or equivalent amounts of rat IgG. All biologics were acquired from BioXcell. Recombinant IFN-γ was acquired from Tonbo.
Zander R.A., Obeng-Adjei N., Guthmiller J.J., Kulu D.I., Li J., Ongoiba A., Traore B., Crompton P.D, & Butler N.S. (2015). PD-1 co-inhibitory and OX40 co-stimulatory crosstalk regulates helper T cell differentiation and anti-Plasmodium humoral immunity. Cell host & microbe, 17(5), 628-641.
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