Tergitol np 9

Manufactured by Merck Group

Sourced in United States

Tergitol™ NP-9 is a non-ionic surfactant manufactured by Merck Group. It is a clear, viscous liquid at room temperature. Tergitol™ NP-9 is used as a wetting agent, emulsifier, and detergent in various industrial and laboratory applications.

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Tergitol (12 citations)

4 protocols using tergitol np 9

Recombinant RSV F Protein Production

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Prefusogenic F (BV1184), postfusion F (BV2129), prefusion F DS (BV2267), Cav1 (BV2279), and DS-Cav1 (BV2280) were produced by infecting Sf9 cells (MOI = 0.5 pfu/cell) with recombinant BV for 67 h at 27 °C. Cells were harvested by centrifugation and detergent-lysed with 25 mM Tris-HCL (pH 8.0), 50 mM NaCl, 0.5% (v/v) Tergitol™ NP-9, and 2.0 µg mL⁻¹ leupeptin (Sigma-Aldrich, St. Louis, MO, USA), then RSV F proteins were extracted from cell membranes. RSV F proteins were purified by a combination of anion exchange, lentil lectin affinity, and cation ion exchange chromatography [30 (link)]. For soluble postfusion F (BV2128), Sf9 cells were infected with recombinant BV for 67 h and supernatants were clarified by centrifugation. RSV F (BV2128) was purified with an immobilized metal affinity column (IMAC) and ion exchange chromatography [31 (link)]. Purified RSV F proteins (0.33-1.5 mg mL⁻¹) were formulated in 25 mM phosphate buffer (pH 6.8), 150 mM NaCl, 0.032% (v/v) polysorbate-80 (PS80), and 1% (w/v) histidine.

Patel N., Tian J.H., Flores R., Jacobson K., Walker M., Portnoff A., Gueber-Xabier M., Massare M.J., Glenn G., Ellingsworth L, & Smith G. (2020). Flexible RSV Prefusogenic Fusion Glycoprotein Exposes Multiple Neutralizing Epitopes that May Collectively Contribute to Protective Immunity. Vaccines, 8(4), 607.

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Formulation of Drug-Loaded Nano-Carriers

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Materials were obtained from Sigma unless otherwise noted. The following were used: Pluronic F127 (Sigma # P2443), Pluronic F108 (Sigma # 542342), Pluronic F68 (Sigma # 412325), Polysorbate 20 (Amresco # M147-1L), Polysorbate 40 (Sigma # 1547936), Polysorbate 80 (VWR # EM-9490), Cremophor EL (Sigma # C5135), Cremophor RH40 (Sigma # 07076), Tergitol NP 9 (Sigma # NP9), Tergitol NP 10 (Sigma # NP10), Tergitol NP 40 (Sigma # NP40S), Brij 97 (Spectrum # B1679), Brij 35 (Spectrum # BR105), Brij L23 (Sigma # 16005), Brij O20 (Sigma # 436240), Vitamin K1 (VK1, VWR # AAAL10575-03), cyclosporine A (CsA, VWR # 89156-334), Testosterone undecanote (T-undec, Matrix # 099258), cholecalciferol (VWR, TCC0314), Retinol Palmitate (VWR # IC15652125), coenzyme Q10 (Sigma # 45-C9538), Docetaxel (LC Labs # D-1000), Paclitaxel (LC Labs # P-9600), Cabazitaxel (CTX, Proactive Molecular Research), Squalene (VWR # AAB20944-22), 5,9,14,18,23,27,32,36-Octabutoxy-2,3-naphthalocyanine (ONc, Sigma # 412074) 2,9,16,23-Tetra-tert-butyl-29H,31H-phthalocyanine (BPc, Sigma # 423157), Zinc 2,11,20,29-tetra-tert-butyl-2,3-naphthalocyanine (ZnBNc, Sigma # 432210), Castor oil (Sigma # 259853), methylene chloride (Fisher), Ethanol (Decon), ethylene glycol (VWR # 1071401), and sodium chloride (Macron # 7532-06).

Zhang Y., Song W., Geng J., Chitgupi U., Unsal H., Federizon J., Rzayev J., Sukumaran D.K., Alexandridis P, & Lovell J.F. (2016). Therapeutic surfactant-stripped frozen micelles. Nature Communications, 7, 11649.

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Synthesis and Characterization of Surfactant-Coated Microparticles

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All chemicals were used as received. 1-bromopentane (99%) (Alfa Aesar); 1-bromohexane (99%) (Alfa Aesar); 1-bromoheptane (>98%) (Toyoko Chemical Industry); 1-bromooctane (>98%) (Toyoko Chemical Industry); 1-bromononane (99%) (Alfa Aesar); 1-bromodecane (>98%) (Toyoko Chemical Industry); 1bromododecane (>98%) (Alfa Aesar); 1-iodohexadecane (>98%, stabilized with copper) (Alfa Aesar); Tergitol NP-9 (Sigma); Tergitol NP-12 (The Dow Chemical Company); Tergitol NP-15 (The Dow Chemical Company); Tergitol NP-30 (The Dow Chemical Company); Makon TD-12 (Stepan Company); polybead carboxylate 1.0 micron microspheres (Polysciences Inc.); 1-(ethoxy)nonafluorobutane, mixture of n-and iso-butyl (Synquest); sodium dodecyl sulfate (Sigma-Aldrich); N-(triethoxysilylpropyl)-o-polyethylene oxide urethane (95%) (Gelest); hexadecyltrimethoxysilane (>85%) (Sigma-Aldrich).

Wentworth C.M., Castonguay A.C., Moerman P.G., Meredith C.H., Balaj R.V., Cheon S.I, & Zarzar L.D. (2022). Chemically Tuning Attractive and Repulsive Interactions between Solubilizing Oil Droplets. Angewandte Chemie (International ed. in English), 61(32).

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Quantification of Luciferase and Nanoluc in Cell Cultures

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Human IFN-γ from Roussel Uclaf (Romainville, France) was a kind gift of Dr. Mounira Chelbi-Alix. IL-2, IL-15, and TGF-β were obtained from Peprotech (Rocky Hill, NJ). R848 was obtained from Sigma-Aldrich, Torin2 was from Tocris (Bristol, UK), and rapamycin was from Calbiochem (San Diego, CA). Luciferase induction in twINNE cells was determined using the Bright-Glo (Promega, Fitchburg, WI) or Britelite plus reagents (PerkinElmer, Waltham, MA), according to the manufacturer's recommendations. Nanoluc expression was quantified in culture supernatants as previously described. 19 Cellular viability was determined by quantification of ATP in culture wells using the CellTiter-Glo assay (Promega). Bioluminescence was measured for 0.1 s with a luminometer (EnSpire; PerkinElmer). Tergitol NP9 was from Sigma-Aldrich.

Hayek S., Bekaddour N., Besson L., Alves de Sousa R., Pietrancosta N., Viel S., Smith N., Jacob Y., Nisole S., Mandal R., Wishart D.S., Walzer T., Herbeuval J.P, & Vidalain P.O. (2019). Identification of Primary Natural Killer Cell Modulators by Chemical Library Screening with a Luciferase-Based Functional Assay. SLAS discovery : advancing life sciences R & D, 24(1).

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