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Anti chmp2a

Manufactured by Proteintech
Sourced in United States

Anti-CHMP2A is a primary antibody product developed and manufactured by Proteintech. It is designed to detect and bind to the CHMP2A protein, which is involved in the endosomal sorting complex required for transport (ESCRT) pathway. The antibody can be used in various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of the CHMP2A protein.

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2 protocols using anti chmp2a

1

Western Blot Analysis of Protein Markers

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Western blotting was performed as previously described [17 (link)]. The primary antibodies used were as follows: anti-PTEN (Abcam Cat# ab32199, RRID:AB_777535; Cell Signaling Technology Cat# 9188, RRID:AB_2253290; Proteintech Cat# 60300-1-Ig, RRID:AB_2881415), anti-NGAL (Abcam Cat# ab63929, RRID:AB_1140965), anti-FN (Sigma-Aldrich Cat# F3648, RRID:AB_476976), anti-Col-I (Boster Biological Technology Cat# BA0325, RRID:AB_2891224), anti-α-SMA (Sigma-Aldrich Cat# A5228, RRID:AB_262054), anti-PCNA (Proteintech Cat# Biotin-60097, RRID:AB_2883063), anti-E-Cadherin (Cell Signaling Technology Cat# 14472, RRID:AB_2728770), anti-Flag (Proteintech Cat# 20543-1-AP, RRID:AB_11232216), anti-CHMP2A (Proteintech Cat# 10477-1-AP, RRID:AB_2079470), anti-SQSTM1/p62 (Cell Signaling Technology Cat# 39749, RRID:AB_2799160), anti-LC3B (Cell Signaling Technology Cat# 83506, RRID:AB_2800018), anti-LAMP1 (Cell Signaling Technology Cat# 15665, RRID:AB_2798750), anti-β-tubulin (Tianjin Sungene Biotech Cat# KM9003, RRID:AB_2744678), and anti-GAPDH (Tianjin Sungene Biotech Cat# KM9002, RRID:AB_2721026). Western blotting was performed at least three times, independently. Quantification was performed by measuring the signal intensity using the software ImageJ (National Institutes of Health).
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2

Comprehensive Antibody Panel for NLRP3 Inflammasome

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The following antibodies were used: anti-NLRP3 (catalog number 15101), anti-Akt (catalog number 4691), anti-phospho-Akt (Ser473) (catalog number 4060), anti-LC3B (catalog number 2775), anti-IL-1β (catalog number 12242), anti-GSDMD (catalog number 39754), anti-cleaved-caspase-1 (Asp296) (catalog number 89332), anti-cleaved-caspase-1 (Asp297) (catalog number 4199), anti-phospho-mTOR (ser2448) (catalog number 5336), and anti-mTOR (catalog number 2972) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA); anti-p62 (catalog number A11250), Alexa Fluor 488 goat anti-rabbit IgG (AS053), and anti-AIM2 (catalog number A3356) were obtained from ABclonal Technology (Wuhan, China); antibodies against ASC (catalog number sc-514414) was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA); and anti-β-actin (catalog number 66009-1-lg) monoclonal antibody and caspase-1 (catalog number 22915-1-AP), and anti-CHMP2A (catalog number 10477-1-AP) were acquired from Protein Tech Group (Chicago, IL).
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