Total LDH activity and serum creatinine were determined by kinetic colorimetric assays on a Cobas® 8000 analyzer (Roche Diagnostics, Mannheim, Germany). In parallel, white blood cell (WBC) counts were determined by an Advia 2120 Hematology System analyzer (Bayer Diagnostics, Tarrytown, NJ, USA). The concentrations of C-reactive protein (CRP), ferritin, and cTnT were determined by electro-chemiluminescent immunoassay (Cobas® e 411 analyzer, Roche Diagnostics), while D-dimer was analyzed by immunoturbidimetry (BCS® XP, Siemens, Munich, Germany). The CKD-EPI (Chronic Kidney Disease Epidemiology Collaboration) equation was used to estimate the glomerular filtrate rate (GFR).
The five isoenzymes of LDH were separated by electrophoresis using LDH Hydragel® 7 kit (Sebia, Norcross, GA, USA) on alkaline buffered (pH 8.4) agarose gel. The separated isoenzymes were visualized using a specific chromogenic substrate, and the amount of formazan precipitate was proportional to the LDH enzymatic activity. A semi-automated HYDRASYS® electrophoresis instrument (Sebia) was applied to obtain gels ready for interpretation. The dried gels were processed for densitometry to achieve an accurate relative quantification of individual zones. Abnormal ratio of LDH isoenzymes was evaluated based on the manufacturer’s instructions of LDH Hydragel® 7 kit.
The five isoenzymes of LDH were separated by electrophoresis using LDH Hydragel® 7 kit (Sebia, Norcross, GA, USA) on alkaline buffered (pH 8.4) agarose gel. The separated isoenzymes were visualized using a specific chromogenic substrate, and the amount of formazan precipitate was proportional to the LDH enzymatic activity. A semi-automated HYDRASYS® electrophoresis instrument (Sebia) was applied to obtain gels ready for interpretation. The dried gels were processed for densitometry to achieve an accurate relative quantification of individual zones. Abnormal ratio of LDH isoenzymes was evaluated based on the manufacturer’s instructions of LDH Hydragel® 7 kit.