Revolution xd spinning disk confocal system

Manufactured by Oxford Instruments

The Revolution XD spinning disk confocal system is a high-performance microscopy solution designed for advanced imaging applications. It features a high-speed spinning disk that enables rapid image acquisition, making it suitable for live-cell imaging and other time-sensitive experiments. The system provides optical sectioning capabilities, allowing for the capture of thin optical slices within a sample, which can be used to reconstruct three-dimensional images. The Revolution XD is engineered to deliver high-quality, high-resolution images with minimal phototoxicity, making it a valuable tool for a wide range of scientific research applications.

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Lab products found in correlation

Smartshutter, by Sutter Instruments (1 mentions) Csu x1 confocal head, by Yokogawa (1 mentions) Ixonem 888, by Oxford Instruments (1 mentions) Perfect focus, by Nikon (1 mentions) Ultra 888 emccd camera, by Oxford Instruments (1 mentions)

Close spelling variants of this product

Andor Revolution XD Spinning Disk Confocal System XD spinning disk confocal system Revolution spinning disk confocal system Spinning disk confocal system Revolution XD System Revolution XD Revolution confocal system

2 protocols using revolution xd spinning disk confocal system

Spinning Disk Confocal Microscopy

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Images were acquired using a Revolution XD spinning disk confocal system (Andor) with a CSU-X1 confocal head (Yokogawa) and mounted on a TE 2000E inverted microscope with Perfect Focus (Nikon). Confocal images were acquired using an Andor iXonEM+ 888 electron-multiplying charge-coupled device camera. Transillumination was provided by a halogen lamp and controlled by a SmartShutter (Sutter Instrument). Confocal excitation was provided by an Andor Laser Combiner with three laser lines at 488, 561, and 647 nm. Emission wavelength was controlled using a Sutter LB10W-2800 filter wheel outfitted with bandpass filters from Chroma Technology. Image acquisition and all other peripherals were controlled by Micro-Manager (https://micro-manager.org/). A Nikon CFI Plan Apo 100×/1.4-NA objective was used. Experiments were performed at room temperature using ASW as imaging medium unless stated otherwise.

Zhang X.F., Ajeti V., Tsai N., Fereydooni A., Burns W., Murrell M., De La Cruz E.M, & Forscher P. (2019). Regulation of axon growth by myosin II–dependent mechanocatalysis of cofilin activity. The Journal of Cell Biology, 218(7), 2329-2349.

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Vesicle Transport Dynamics Quantification

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Live imaging was performed using an IX83 Andor Revolution XD Spinning Disk Confocal System with an environmental chamber at 37°C, a 60x oil objective (NA 1.30) and a 2x magnifier coupled to an iXon Ultra 888 EMCCD Camera. Images were taken at the rate of 1 frame/sec for 3 or 5 minutes. Kymographs were generated with ImageJ (NIH) to quantify the distance travelled, velocity and direction of transport of the individual vesicles. Individual runs were counted if the pause between two processive movements was less than 10 seconds. If the pause was longer, then the runs were counted as separate.

Khobrekar N.V., Quintremil S., Dantas T.J, & Vallee R.B. (2020). The Dynein Adaptor RILP Controls Neuronal Autophagosome Biogenesis, Transport and Clearance. Developmental cell, 53(2), 141-153.e4.

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