Mir 206 inhibitor

The shRNA MALAT1 (sh-MALAT1), sh-negative control (sh-NC), miR-206 inhibitor, inhibitor NC, miR-206 mimics, miR-NC, sh-ARNT and sh-PPARα were obtained from GenePharma (Shanghai, China). HepG2 cells transfected with sh-MALAT1, sh-NC, miR-206 inhibitor, inhibitor NC, miR-206 mimics, miR-NC, sh-ARNT or sh-PPARα by using Lipofectamine 3000 (Invitrogen).

HepG2/Huh7 cells were transfected with miR-206 mimic or scramble control miR-NC (GenePharma, Shanghai, China) or miR-206 inhibitor (Invitrogen) using Lipofectamine 2000 (Invitrogen) following the manufacturer's protocol. Cells were cultured for 48 h before analysis or other subsequent experiments. PTP1B overexpression vector was constructed by cloning human PTP1B cDNA into pMSCV-hygro vector using the following primers: 5′-ATGGAGATGGAAAAGGAGTTCGA-3′ and 5′-TGTGTTGCTGTTGAACAGGAA-3′. The pMSCV-hygro-PTP1B clones were sequencing verified. HepG2 and Huh7 cells were transfected with pMSCV-PTP1B vector and selected with G418 for 3–4 weeks. Monoclonal cells were then selected and examined for PTP1B overexpression.