Autostainer 3400

Manufactured by Agilent Technologies

The Autostainer 3400 is an automated slide staining instrument designed for immunohistochemistry (IHC) and in situ hybridization (ISH) applications in clinical and research laboratories. It performs standardized staining protocols with consistency and efficiency.

Automatically generated - may contain errors

Lab products found in correlation

Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Dmd108, by Leica (1 mentions) Olig2, by Abcam (1 mentions) Eclipse t2000 e, by Nikon (1 mentions) Alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Idh1 r132h, by Dianova (1 mentions) Lsab kit, by Agilent Technologies (1 mentions)

2 protocols using autostainer 3400

Immunohistochemical Staining for Glioma Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Standard H&E and immunofluorescent staining procedures were performed. The Duke University Pathology Research Histology and Immunohistochemistry Laboratory processed and stained samples for IHC. A standard protocol requiring antigen retrieval in citrate buffer (pH 6.0, 80° Celsius) was used. For IHC, the DAKO Autostainer 3400 was used. Antibodies included: β(III)Tubulin 1:1000 Covance-MMS-435P), CD31 1:100 (Abcam-ab28364), IDH1-R132H 1:100 (Dianova-H09), Ki67 1:100 (BD Pharm-550609), Olig2 1:500 (Abcam-ab136253), Sox2 1:100 (StemCell Technologies-60055). Secondary antibodies included: Alexa Fluor 488 (ThermoFisher) and Alexa Fluor 594 (ThermoFisher). Immunofluorescent staining was performed in accordance with the aforementioned StemCell Technologies kit. H&E and IHC samples were imaged using the Leica DMD 108. Immunofluorescent images were imaged on the Nikon Eclipse T2000-E. Positively stained cells were both manually counted and counted with ImageJ. Those assigned to counting were blinded to animal genotype and were given designated quadrants of specific size and magnification to count.

Pirozzi C.J., Carpenter A.B., Waitkus M.S., Wang C.Y., Zhu H., Hansen L.J., Chen L.H., Greer P.K., Feng J., Wang Y., Bock C.B., Fan P., Spasojevic I., McLendon R.E., Bigner D.D., He Y, & Yan H. (2017). Mutant IDH1 Disrupts the Mouse Subventricular Zone and Alters Brain Tumor Progression. Molecular cancer research : MCR, 15(5), 507-520.

+ Open protocol

+ Expand

Quantifying Eosinophils and Mast Cells in Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Eosinophil density was determined from the routine histology slides (hematoxylin and eosin stain) by counting eosinophils beginning in what appeared to be the most involved area after scanning the entire specimen. Five consecutive fields (400× magnification) were evaluated. Peak count was defined as the highest count of the five fields, while mean count was defined as the average of the five fields.
Mast cell density was evaluated utilizing immunohistochemical techniques. Serial 3-μm paraffin sections were air dried and heat fixed on slides. The sections were deparaffinized with xylene and iodine and rehydrated in a graded series of alcohol. Sections were stained on an automated Dako Autostainer 3400 using Dako’s LSAB + kit with streptoavidin conjugated to horseradish peroxidase. The antibody utilized was tryptase monoclonal mouse antihuman mast cell tryptase, clone AA1, Dako. Mast cell density was determined by counting mast cells beginning in what appeared to be the most involved area after scanning the entire specimen. Five consecutive fields (400× magnification) were evaluated. Peak count was defined as the highest count of the five fields, while mean count defined as the average of the five fields.

Neilan N.A., Garg U.C., Schurman J.V, & Friesen C.A. (2014). Intestinal permeability in children/adolescents with functional dyspepsia. BMC Research Notes, 7, 275.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!